A widespread family of phage-inducible chromosomal islands only steals bacteriophage tails to spread in nature
A widespread family of phage-inducible chromosomal islands only steals bacteriophage tails to spread in nature
复制标题
一个广泛存在的噬菌体诱导染色体岛家族仅窃取噬菌体尾巴以在自然界中传播
DOI：
10.1101/2022.09.08.507074
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Alqurainy N
中科院分区：
文献类型：
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作者：
Alqurainy N
噬菌体卫星是将它们的生命周期与它们寄生的辅助噬菌体的生命周期相结合的遗传元件，通过产生小衣壳来干扰噬菌体包装，其中只有卫星被包装。到目前为止，在所有分析的系统中，卫星大小的衣壳由噬菌体蛋白组成。在这里，我们报告说，一个家庭的噬菌体诱导的染色体岛（PICI），一种卫星，编码的所有蛋白质都需要生产小尺寸的衣壳和独家包装的PICI到这些衣壳。因此，这个新的家族，命名为captoblast-forming PICI（cf-PICI），只需要噬菌体尾部产生PICI颗粒。值得注意的是，代表性的cf-PICI是从它们的辅助分子中无成本产生的，这表明这些元素之间的关系不是寄生的。最后，我们的基因组研究表明，cf-PICI存在于革兰氏阳性和革兰氏阴性细菌中，并且已经独立进化了至少三次以在自然界中传播。
Phage satellites are genetic elements that couple their life cycle to that of helper phages they parasitize, interfering with phage packaging through the production of small capsids, where only satellites are packaged. So far, in all analyzed systems, the satellite-sized capsids are composed of phage proteins. Here, we report that a family of phage-inducible chromosomal islands (PICIs), a type of satellites, encodes all the proteins required for both the production of small-sized capsids and the exclusive packaging of the PICIs into these capsids. Therefore, this new family, named capsid-forming PICIs (cf-PICIs), only requires phage tails to generate PICI particles. Remarkably, the representative cf-PICIs are produced with no cost from their helper phages, suggesting that the relationship between these elements is not parasitic. Finally, our phylogenomic studies indicate that cf-PICIs are present both in gram-positive and gram-negative bacteria and have evolved at least three times independently to spread in nature.
影响因子：
3.7
作者：
Hoffmann S;Schmidt C;Walter S;Bender JK;Gerlach RG
通讯作者：
Gerlach RG