Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis.
Pattern of nonspecific (or global) DNA methylation in oral carcinogenesis.
复制标题
口腔癌发生中非特异性（或整体）DNA 甲基化的模式。
DOI：
10.1002/hed.20288
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Grizzle,WilliamE
中科院分区：
文献类型：
--
作者：
Piyathilake,ChandrikaJ;Bell,WalterC;Jones,Jennifer;Henao,OlgaL;Heimburger,DouglasC;Niveleau,Alain;Grizzle,WilliamE
BackgroundAlthough alterations in nonspecific (or global) DNA methylation (GDM) in specific cells are known to be involved in the process of lung carcinogenesis, similar associations have not been evaluated in other smoking‐related cancers of the head and neck.MethodsWe evaluated the status of GDM by using monoclonal antibodies specific for 5‐methylcytosine (5‐mc) in oral squamous cell carcinoma (SCC) specimens of 48 cigarette smokers who had SCC develop and in 93 age‐, race‐, and sex‐matched smokers who did not.ResultsPercentages of cells positive for 5‐mc immunostaining of DNA of SCC and dysplastic lesions were significantly higher than those of normal oral epithelial cells from cancer subjects and from noncancer subjects. The degree of DNA methylation was unrelated to DNA content.ConclusionsThe pattern of GDM in oral SCCs is different from that of lung SCCs. The differences in nutrient risk factor profiles that are related to GDM and differential activity of DNA methyltranferases between oral and lung SCCs may explain these observations. © 2005 Wiley Periodicals, Inc.Head Neck27:XXX–XXX, 2005
