Establishment of a Chinese bladder cancer cell line (T921) with high metastatic activity
Establishment of a Chinese bladder cancer cell line (T921) with high metastatic activity
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高转移活性中国膀胱癌细胞系（T921）的建立
DOI：
10.1007/s11626-013-9651-z
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发表时间：
2013-10-01
影响因子：
2.1
通讯作者：
Sun, Yinghao
中科院分区：
文献类型：
--
作者：
Xu, Chuanliang;Xu, Weidong;Sun, Yinghao
肌肉浸润性膀胱癌在没有标准器官偏好的情况下容易发生转移。目前用于研究膀胱癌的细胞系主要来自西方人群，尚未从中国人群中建立人膀胱癌细胞系。膀胱癌细胞系源自一名患有肌层浸润性膀胱癌的中国女性患者，然后将这些细胞异种移植到三只裸鼠的膀胱中。五周后，处死这些小鼠以观察局部侵袭和远处转移。转移性肿瘤也被切除并分析以评估转移机制。核型和免疫组织化学分析证明，该膀胱癌细胞系命名为T921，已成功建立。细胞系原位转移 5 周后，所有三只裸鼠均观察到多器官转移。此外，上皮-间质转化（EMT）相关基因也参与肿瘤转移。成功建立T921膀胱癌细胞系，观察EMT在膀胱癌转移中的作用。
Muscle-invasive bladder cancer is prone to metastasis without a standard organ preference. The current cell lines used to study bladder cancer have primarily been derived from individuals in Western populations, and no human bladder cancer cell line has been established from the Chinese population. A bladder cancer cell line was derived from a female Chinese patient with muscle-invasive bladder cancer, and these cells were then xenografted into the bladders of three nude mice. Five weeks later, these mice were killed to observe local invasion and distant metastasis. The metastatic tumors were also removed and analyzed to assess the metastatic mechanism. This bladder cancer cell line, named T921, was successfully established, as evidenced by karyotype and immunohistochemistry analyses. Multi-organ metastases were observed in all three of the nude mice 5 wk after the orthotopic transfer of the cell line. In addition, epithelial–mesenchymal transition (EMT)-related genes were involved in the tumor metastases. The T921 bladder cancer cell line was successfully established, and EMT was observed to play a role in bladder cancer metastasis.