Metabolic switching is impaired by aging and facilitated by ketosis independent of glycogen
Metabolic switching is impaired by aging and facilitated by ketosis independent of glycogen
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DOI：
10.18632/aging.103116
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发表时间：
2020-05-15
期刊：
影响因子：
5.2
通讯作者：
Burke, Sara
中科院分区：
文献类型：
--
作者：
Hernandez, Abbi;Truckenbrod, Leah;Burke, Sara
在糖酵解和酮症之间转换的能力通过在禁食期间通过脂肪氧化进行代谢来促进生存。碳水化合物限制或压力也可以引起代谢转换。在老年大鼠中，糖酵解的酮适应延迟，但介导这种与年龄相关的损害的因素尚未确定。我们测量了糖酵解和酮症之间的代谢转换，以及糖原动力学，在年轻和老年大鼠进行时间限制喂养（TRF）与标准饮食或低碳水化合物生酮饮食（KD）。TRF单独逆转胰岛素相关代谢缺陷的标志物，并加速老年动物的代谢转换。然而，KD+TRF在这些变量上提供了额外的好处。值得注意的是，酮适应能力与糖原水平无关，KD喂养的大鼠在肾上腺素给药后葡萄糖升高。这项研究为酮适应机制提供了新的见解，证明了饮食干预在整个生命周期中治疗代谢障碍的效用。
The ability to switch between glycolysis and ketosis promotes survival by enabling metabolism through fat oxidation during periods of fasting. Carbohydrate restriction or stress can also elicit metabolic switching. Keto-adapting from glycolysis is delayed in aged rats, but factors mediating this age-related impairment have not been identified. We measured metabolic switching between glycolysis and ketosis, as well as glycogen dynamics, in young and aged rats undergoing time-restricted feeding (TRF) with a standard diet or a low carbohydrate ketogenic diet (KD). TRF alone reversed markers of insulin-related metabolic deficits and accelerated metabolic switching in aged animals. A KD+TRF, however, provided additive benefits on these variables. Remarkably, the ability to keto-adapt was not related to glycogen levels and KD-fed rats showed an enhanced elevation in glucose following epinephrine administration. This study provides new insights into the mechanisms of keto-adaptation demonstrating the utility of dietary interventions to treat metabolic impairments across the lifespan.