RIBOSOMAL DNA AND STELLATE GENE COPY NUMBER VARIATION ON THE Y-CHROMOSOME OF DROSOPHILA-MELANOGASTER
RIBOSOMAL DNA AND STELLATE GENE COPY NUMBER VARIATION ON THE Y-CHROMOSOME OF DROSOPHILA-MELANOGASTER
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DOI：
10.1073/pnas.86.6.1944
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发表时间：
1989-03-01
影响因子：
11.1
通讯作者：
CLARK, AG
中科院分区：
文献类型：
--
作者：
LYCKEGAARD, EMS;CLARK, AG
Y染色体上的多基因家族面临着一系列不同寻常的进化力量。核糖体DNA和星状体，这里研究的两个家族，在X和Y染色体上都有多个相似序列的拷贝。虽然Y染色体上的序列趋异率取决于突变率、基因转换和与X染色体的交换，以及纯化选择，但基因拷贝数的调节也可能取决于其他多效性功能，例如染色体配对的维持。基因拷贝数估计了一系列的34个Y染色体置换线使用密度测量的狭缝印迹的基因组DNA从成年果蝇。用克隆的乙醇脱氢酶基因（单拷贝基因）探测的相同印迹的放射自显影扫描图作为内标。核糖体DNA的拷贝数跨越6倍范围，星状DNA的拷贝数跨越3倍范围。尽管这种变化的幅度，有没有之间的关联拷贝数和分离的性染色体的变化。
Multigene families on the Y chromosome face an unusual array of evolutionary forces. Both ribosomal DNA and Stellate, the two families examined here, have multiple copies of similar sequences on the X and Y chromosomes. Although the rate of sequence divergence on the Y chromosome depends on rates of mutation, gene conversion and exchange with the X chromosome, as well as purifying selection, the regulation of gene copy number may also depend on other pleiotropic functions, such as maintenance of chromosome pairing. Gene copy numbers were estimated for a series of 34 Y chromosome replacement lines using densitometric measurements of slot blots of genomic DNA from adult Drosopholia melanogaster. Scans of autoradiographs of the same blots probed with the cloned alcohol dehydrogenase gene, a single copy gene, served as internal standards. Copy numbers span a 6-fold range for ribosomal DNA and a 3-fold range for Stellate DNA. Despite this magnitude of variation, there was no association between copy number and segregation variation of the sex chromosomes.