Characterization of the Large Picornaviral Polypeptides Produced in the Presence of Zinc Ion
Characterization of the Large Picornaviral Polypeptides Produced in the Presence of Zinc Ion
复制标题
锌离子存在下产生的大小核糖核酸病毒多肽的表征
作者：
B. E. Butterworth;B. Korant
锌离子抑制人鼻病毒-1A型、脑心肌炎病毒和脊髓灰质炎病毒多肽的翻译后切割。每种病毒对锌的易感性不同。然而，在每一种情况下，脑心肌炎病毒衣壳前体的裂解和类似于C→D→E转换的裂解对锌最敏感。较高的锌浓度会导致更大的前体多肽的积聚，大小在106,000到214,000道尔顿之间。测定了这些多肽的大小及其基因座在病毒RNA上的相对位置。这些数据被用来将这些多肽放在病毒蛋白加工的总体方案中。
Zinc ion inhibits the posttranslational cleavages of human rhinovirus-1A, encephalomyocarditis virus, and poliovirus polypeptides. Each virus displayed a different susceptibility to zinc. However, in each case the cleavages of the capsid precursor and the cleavages analogous to the C → D → E conversion in encephalomyocarditis virus were most sensitive to zinc. Higher concentrations of zinc resulted in the buildup of even larger precursor polypeptides of a size between 106,000 and 214,000 daltons. The sizes of these polypeptides and the relative position of their gene loci on the viral RNA were determined. These data were used to place these polypeptides in the over-all scheme of viral protein processing.