Description of Lactobacillus iwatensis sp. nov., isolated from orchardgrass (Dactylis glomerata L.) silage, and Lactobacillus backii sp. nov.
Description of Lactobacillus iwatensis sp. nov., isolated from orchardgrass (Dactylis glomerata L.) silage, and Lactobacillus backii sp. nov.
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DOI：
10.1099/ijs.0.051920-0
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发表时间：
2013-10
影响因子：
2.8
通讯作者：
M. Tohno;M. Kitahara;T. Irisawa;T. Masuda;R. Uegaki;M. Ohkuma;K. Tajima
中科院分区：
文献类型：
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作者：
M. Tohno;M. Kitahara;T. Irisawa;T. Masuda;R. Uegaki;M. Ohkuma;K. Tajima
从鸭茅（Dactylis glomerata L.）来自日本岩手县的青贮饲料，并进行了分类研究。两种微生物均为杆状、革兰氏染色阳性、过氧化氢酶阴性、兼性厌氧和同型发酵。细胞壁不含内消旋二氨基庚二酸，主要脂肪酸为C18：1ω 9 c和C19 cyclo 9，10/：1。16 S rRNA、pheS和rpoA基因序列的比较分析表明，这些菌株是新的，属于乳杆菌属。基于16 S rRNA基因序列相似性，分离物与乳杆菌属的以下成员的模式菌株最密切相关：棒状乳杆菌亚种（Lactobacillus coryniformis subsp. coryniformis（96.7%相似性）、L.棒形亚种torquens（96.6%），L.双酶链霉菌（95.5%）和L.凝乳酶（94.1%）。然而，IWT 246（T）和IWT 248两者的16 S rRNA基因序列与“巴氏乳杆菌”JCM 18665的16 S rRNA基因序列有99.7%的相似性;该名称尚未有效地发表。基因型，表型和化学分类学分析证实，这些新的菌株占据了独特的分类地位。DNA-DNA杂交实验证明了新分离物与相关乳杆菌属物种的基因型分离。新的分离株命名为Iwatensis Lactobacillus sp. nov.，以菌株IWT 246（T）（= JCM 18838（T）= DSM 26942（T））为模式菌株。我们的研究结果还表明，'L。巴克氏菌确实代表了一种新的乳杆菌属物种。细胞在其细胞壁肽聚糖中不含内消旋二氨基庚二酸，主要脂肪酸为C16：0、C19环9，10/：1和总特征10（C18：1ω 11 c、C18：1ω 9 t、C18：1ω 6 t和未知ECL 17.834中的一种或多种）。因此，我们建议将其更正为巴克氏乳杆菌（Lactobacillus backii sp. nov.），模式菌株JCM 18665（T）（= LMG 23555（T）= DSM 18080（T）= L1062（T））。
Two bacterial strains, designated IWT246(T) and IWT248, were isolated from orchardgrass (Dactylis glomerata L.) silage from Iwate prefecture, Japan, and examined for a taxonomic study. Both organisms were rod-shaped, Gram-stain-positive, catalase-negative, facultatively anaerobic and homofermentative. The cell wall did not contain meso-diaminopimelic acid and the major fatty acids were C18 : 1ω9c and C19 cyclo 9,10/:1. Comparative analyses of 16S rRNA, pheS and rpoA gene sequences revealed that these strains were novel and belonged to the genus Lactobacillus. Based on 16S rRNA gene sequence similarity, the isolates were most closely related to the type strains of the following members of the genus Lactobacillus: Lactobacillus coryniformis subsp. coryniformis (96.7 % similarity), L. coryniformis subsp. torquens (96.6 %), L. bifermentans (95.5 %) and L. rennini (94.1 %). However, the 16S rRNA gene sequences of both IWT246(T) and IWT248 were 99.7 % similar to that of 'Lactobacillus backi' JCM 18665; this name has not been validly published. Genotypic, phenotypic and chemotaxonomic analyses confirmed that these novel strains occupy a unique taxonomic position. DNA-DNA hybridization experiments demonstrated genotypic separation of the novel isolates from related Lactobacillus species. The name Lactobacillus iwatensis sp. nov. is proposed for the novel isolates, with strain IWT246(T) ( = JCM 18838(T) = DSM 26942(T)) as the type strain. Our results also suggest that 'L. backi' does represent a novel Lactobacillus species. The cells did not contain meso-diaminopimelic acid in their cell-wall peptidoglycan and the major fatty acids were C16 : 0, C19 cyclo 9,10/:1 and summed feature 10 (one or more of C18 : 1ω11c, C18 : 1ω9t, C18 : 1ω6t and unknown ECL 17.834). We therefore propose the corrected name Lactobacillus backii sp. nov., with the type strain JCM 18665(T) ( = LMG 23555(T) = DSM 18080(T) = L1062(T)).