Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise
Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise
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DOI：
10.1086/315217
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发表时间：
2000-01-01
影响因子：
6.4
通讯作者：
Stell, AL
中科院分区：
文献类型：
--
作者：
Johnson, JR;Stell, AL
采用新型聚合酶链反应（PCR）方法，对75株尿毒症大肠埃希菌进行了29个毒力因子（VF）基因的检测。与探针杂交法相比，PCR法的特异性为100%，灵敏度为97.1%。fyuA （yersiniabactin：总患病率，93%）、traT（血清耐药性，68%）和致病性相关岛标记物（71%）发生在来自受损和未受损宿主的大多数菌株中。出现在
Among 75 urosepsis isolates of Escherichia coli, 29 virulence factor (VF) genes were detected by use of a novel polymerase chain reaction (PCR) assay. Compared with probe hybridization, the PCR assay's specificity was 100% and sensitivity 97.1%. fyuA (yersiniabactin: overall prevalence, 93%), traT (serum resistance, 68%), and a pathogenicity-associated island marker (71%) occurred in most strains from both compromised and noncompromised hosts. Present in