Regular Endurance Exercise Promotes Fission, Mitophagy, and Oxidative Phosphorylation in Human Skeletal Muscle Independently of Age
Regular Endurance Exercise Promotes Fission, Mitophagy, and Oxidative Phosphorylation in Human Skeletal Muscle Independently of Age
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DOI：
10.3389/fphys.2019.01088
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发表时间：
2019-08-22
影响因子：
4
通讯作者：
Deldicque, Louise
中科院分区：
文献类型：
--
作者：
Balan, Estelle;Schwalm, Celine;Deldicque, Louise
本研究调查了是否定期耐力运动保持基础线粒体自噬和线粒体功能在衰老过程中。线粒体蛋白质和总mRNA分离股外侧肌活检（n = 33）的年轻久坐（YS），老年久坐（OS），年轻活跃（YA），老年活跃（OA）男子。使用qRT-PCR、蛋白质印迹和免疫荧光染色评估线粒体自噬、分裂、融合、有丝分裂和线粒体代谢的标志物。与年龄无关，活动组的裂变蛋白Fist高于久坐组（+80%; P < 0.05）。线粒体自噬蛋白PARKIN在OA中比在OS中更高（+145%; P = 0.0026）。自噬体合成相关基因Beclin 1和Gabarap mRNA在OS中的表达低于YS和OA（P < 0.05）。OA组融合蛋白和氧化磷酸化蛋白水平高于OS组（P < 0.05），而考克斯活性仅OA组高于OS组（P = 0.032）。有丝分裂的转录调控并不随年龄或运动而变化。总之，身体活跃的生活方式似乎参与了终身线粒体的维护！通过增加分裂和自噬来控制质量。
This study investigated whether regular endurance exercise maintains basal mitophagy and mitochondrial function during aging. Mitochondrial proteins and total mRNA were isolated from vastus lateralis biopsies (n = 33) of young sedentary (YS), old sedentary (OS), young active (YA), and old active (OA) men. Markers for mitophagy, fission, fusion, mitogenesis, and mitochondrial metabolism were assessed using qRT-PCR, Western blot, and immunofluorescence staining. Independently of age, fission protein Fist was higher in active vs. sedentary subjects (+80%; P < 0.05). Mitophagy protein PARKIN was more elevated in OA than in OS (+145%; P = 0.0026). mRNA expression of Beclin1 and Gabarap, involved in autophagosomes synthesis, were lower in OS compared to YS and OA (P < 0.05). Fusion and oxidative phosphorylation proteins were globally more elevated in the active groups (P < 0.05), while COx activity was only higher in OA than in OS (P = 0.032). Transcriptional regulation of mitogenesis did not vary with age or exercise. In conclusion, physically active lifestyle seems to participate in the maintenance of lifelong mitochondria! quality control by increasing fission and mitophagy.