Quantitative analysis of 6985 digitized trypsin G ‐banded human metaphase chromosomes
Quantitative analysis of 6985 digitized trypsin G ‐banded human metaphase chromosomes
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6985条数字化胰蛋白酶G带人类中期染色体的定量分析
DOI：
10.1111/j.1399-0004.1980.tb02296.x
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发表时间：
1980
影响因子：
3.5
通讯作者：
E. Granum
中科院分区：
文献类型：
--
作者：
Claes Lundsieen;J. Philip;E. Granum
在6985条胰蛋白酶G显带的人染色体中测量暗带的频率和染色强度，通过所谓的带转移序列描述，其以浓缩的定量方式代表染色体带型。在单倍体染色体补体中，最多记录了351条带：181条白色带和170条深色带。染色体类型之间条带出现的频率和条带的染色强度差异很大。在深色条带中，深色条带比浅色条带出现的频率更高。对染色体收缩程度和带出现频率之间关系的研究表明，对于所有染色体类型，平均带频率随染色体伸长的增加而增加。巴黎会议（1971年）选择的23条暗带中有20条出现频率高，染色强度高。其余三个标志出现频率较低，分别是由于深色和白色条带融合所致。
The frequency and staining intensity of the dark bands were measured in 6985 trypsin G‐banded human chromosomes, described by so‐called band transition sequences which represent the chromosome banding patterns in a condensed quantitative way. In the haploid chromosome complement a maximum of 351 bands were registered: 181 white and 170 dark bands. The frequency with which bands occurred and the staining intensity of the bands differed considerably between the chromosome types. Among the dark bands the darker stained bands occurred more frequently than the lighter stained bands. A study of the relationship between the degree of chromosome contraction and the frequency of band occurrence revealed that for all chromosome types the average band frequency increased with increasing chromosome elongation. Twenty of the 23 dark bands chosen as landmarks by the Paris Conference (1971) occurred with high frequency and staining intensity. The remaining three landmarks occurred less frequently, due to fusion of dark and white bands, respectively.