SYNTHESIS OF THE NUCLEAR-PROTEIN CYCLIN (PCNA) AND ITS RELATIONSHIP WITH DNA-REPLICATION
SYNTHESIS OF THE NUCLEAR-PROTEIN CYCLIN (PCNA) AND ITS RELATIONSHIP WITH DNA-REPLICATION
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DOI：
10.1016/0014-4827(86)90059-5
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发表时间：
1986-04-01
影响因子：
3.7
通讯作者：
BRAVO, R
中科院分区：
文献类型：
--
作者：
BRAVO, R
血清和纯化的生长因子协同诱导静止小鼠成纤维细胞核蛋白细胞周期蛋白（MW 36000）和DNA的合成。在血清刺激的静止细胞中，由羟基脲或阿非迪霉素抑制DNA合成不影响细胞周期蛋白的诱导。细胞周期蛋白的合成水平在S期结束时迅速下降。免疫荧光研究表明，细胞周期蛋白在细胞核内的分布在S期发生了显著的变化，这些变化取决于DNA合成或S期发生的事件。这些观察结果加强了这样一个概念，即细胞周期蛋白是导致DNA复制的事件的重要组成部分。
Synthesis of the nuclear protein cyclin (MW 36000) and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. Inhibition of DNA synthesis by hydroxyurea or aphidicolin in serum-stimulated quiescent cells does not affect the induction of cyclin. The levels of cyclin synthesis decrease rapidly at the end of the S phase. Immunofluorescence studies reveal that there are dramatic changes in the nuclear distribution of cyclin during S phase and that these depend on DNA synthesis or events during S phase. These observations strengthen the notion that cyclin is an important component of the events leading to DNA replication.