Genome wide expression analysis of the CCR4-NOT complex indicates that it consists of three modules with the NOT module controlling SAGA-responsive genes
Genome wide expression analysis of the CCR4-NOT complex indicates that it consists of three modules with the NOT module controlling SAGA-responsive genes
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DOI：
10.1007/s00438-007-0314-1
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发表时间：
2008-04-01
影响因子：
3.1
通讯作者：
Denis, Clyde L.
中科院分区：
文献类型：
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作者：
Cui, Yajun;Ramnarain, Deepti B.;Denis, Clyde L.
在CCR 4-NOT复合物的9个已知成员中，CCR 4/CAF 1在mRNA死烯化中最重要，而NOT 1 -5蛋白对转录抑制最关键。使用CCR 4-NOT基因中的七个中的缺失的全基因组微阵列分析用于确定受酵母CCR 4-NOT复合物的成员影响的总体mRNA表达模式。在葡萄糖条件下，ccr 4和caf 1在影响基因表达的方式上表现出高度的相似性。与此相反，not缺失在影响基因的方式上是相似的，但与ccr 4/caf 1没有相关性。许多功能相关的蛋白质组由CCR 4/CAF 1或NOT模块特异性控制。重要的是，NOT蛋白优先影响SAGA控制的基因表达。此外，CCR 4/CAF 1和NOT蛋白组在葡萄糖剥夺应激期间对基因表达的影响方面具有更大的相似性。BTT 1是结合核糖体的新生多肽缔合复合物的成员，被证明是CCR 4-NOT复合物的第十个成员，通过CAF 130相互作用。微阵列分析表明，BTT 1和CAF 130在基因表达控制方面高度相关，并优先抑制参与核糖体生物合成的基因。这些结果表明CCR 4-NOT复合物的不同部分控制许多不同的细胞过程。
Of the nine known members of the CCR4-NOT complex, CCR4/CAF1 are most important in mRNA dead-enylation whereas the NOT1-5 proteins are most critical for transcriptional repression. Whole genome microarray analysis using deletions in seven of the CCR4-NOT genes was used to determine the overall mRNA expression patterns that are affected by members of the yeast CCR4-NOT complex. Under glucose conditions, ccr4 and caf1 displayed a high degree of similarity in the manner that they affected gene expression. In contrast, the not deletions were similar in the way they affected genes, but showed no correlation with that of ccr4/caf1. A number of groups of functionally related proteins were specifically controlled by the CCR4/ CAF1 or NOT modules. Importantly, the NOT proteins preferentially affected SAGA-controlled gene expression. Also, both the CCR4/CAF1 and NOT group of proteins shared much greater similarities in their effects on gene expression during the stress of glucose deprivation. BTT1, a member of the nascent polypeptide association complex that binds the ribosome, was shown to be a tenth member of the CCR4-NOT complex, interacting through CAF130. Microarray analysis indicated that BTT1 and CAF130 correlate very highly in their control of gene expression and preferentially repress genes involved in ribosome biogenesis. These results indicate that distinct portions of the CCR4-NOT complex control a number of different cellular processes.