Isolation and characterization of 12 microsatellite loci in the Chinese crocodile lizard (Shinisaurus crocodilurus)
Isolation and characterization of 12 microsatellite loci in the Chinese crocodile lizard (Shinisaurus crocodilurus)
复制标题
中华鳄蜥 (Shinisaurus crocodilurus) 12 个微卫星位点的分离和表征
DOI：
10.1007/s12686-012-9635-4
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发表时间：
2012-09-01
影响因子：
1.1
通讯作者：
Wu, Zhengjun
中科院分区：
文献类型：
--
作者：
Bei, Rongbing;Chen, Jinping;Wu, Zhengjun
中国鳄蜥(Shinisaurus Crocodiurus)被列为世界最濒危物种之一。中国分布的鳄蜥只有9个孤立种群，越南分布有1个种群。从DNA文库中分离并鉴定了12个多态微卫星座位。对来自广东的40只鳄蜥中国个体进行了基因分型，以确定每个座位的特征。这些座位的等位基因数在11~20个之间，观察到的杂合度范围为0.8500~0.9750，期望杂合度范围为0.8372~0.9347。经Bonferroni校正后，所有基因座均符合Hardy-Weinberg平衡。没有发现在任何基因座之间存在显著连锁不平衡的证据。因此，我们期望这些标记将对中国鳄蜥的保护遗传学研究有所帮助。
The Chinese crocodile lizard (Shinisaurus crocodilurus) is listed as one of the most endangered species in the world. There are only nine isolated populations of crocodile lizards distributed in China and one population distributed in Vietnam. 12 polymorphic microsatellite loci were isolated and characterized from a DNA library. To characterize each loci, 40 crocodile lizards individual from Guangdong province, China were genotyped. These loci showed allele numbers ranging from 11 to 20. The ranges of observed and expected heterozygosity were 0.8500–0.9750 and 0.8372–0.9347, respectively. All loci followed Hardy–Weinberg equilibrium (HWE) after Bonferroni correction. No evidence of significant linkage disequilibrium was found among any loci. Thus, we expect these markers will be useful for conservation genetic study of the Chinese crocodile lizard.