Cowhage-induced itch as an experimental model for pruritus. A comparative study with histamine-induced itch.
Cowhage-induced itch as an experimental model for pruritus. A comparative study with histamine-induced itch.
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DOI：
10.1371/journal.pone.0017786
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发表时间：
2011-03-14
期刊：
影响因子：
3.7
通讯作者：
Yosipovitch G
中科院分区：
文献类型：
--
作者：
Papoiu AD;Tey HL;Coghill RC;Wang H;Yosipovitch G
组胺是实验性瘙痒诱导中使用的典型瘙痒原。然而，在大多数慢性瘙痒性疾病中，瘙痒主要不是由组胺介导的。另一方面，奶牛引起的瘙痒似乎更具有慢性皮炎疾病瘙痒的特征。我们测试的有效性，作为一个瘙痒诱导剂，通过对比它与经典的瘙痒诱导剂，组胺，在健康受试者和特应性皮炎（AD）患者的母牛。我们还研究了两种药物联合使用时是否存在累积效应。招募了15名健康个体和15名AD患者。在前臂掌侧的无湿疹区域中进行实验性瘙痒诱导，使用不同的瘙痒诱导剂：组胺、牛油果及其组合。使用计算机辅助视觉模拟量表（COVAS）在刺激应用后连续评估瘙痒强度5.5分钟。在健康和AD受试者中，与组胺相比，施用牛皮后瘙痒的平均和峰值强度更高，并且与单独的组胺相比，在牛皮和组胺组合施用后更高（在所有情况下p<0.0001）。对于使用的相同瘙痒诱导剂，健康受试者和AD受试者之间的瘙痒强度等级没有显著差异;然而，与健康受试者相比，AD受试者表现出延长的瘙痒反应（p<0.001）。与组胺相比，牛胆引起更强烈的瘙痒感。当同时刺激两条通路时，养牛是瘙痒感知的主导因素。奶牛致痒模型是研究AD及其他慢性瘙痒性疾病瘙痒的理想模型，也可作为抗瘙痒药物的人体试验新模型。
Histamine is the prototypical pruritogen used in experimental itch induction. However, in most chronic pruritic diseases, itch is not predominantly mediated by histamine. Cowhage-induced itch, on the other hand, seems more characteristic of itch occurring in chronic pruritic diseases. We tested the validity of cowhage as an itch-inducing agent by contrasting it with the classical itch inducer, histamine, in healthy subjects and atopic dermatitis (AD) patients. We also investigated whether there was a cumulative effect when both agents were combined. Fifteen healthy individuals and fifteen AD patients were recruited. Experimental itch induction was performed in eczema-free areas on the volar aspects of the forearm, using different itch inducers: histamine, cowhage and their combination thereof. Itch intensity was assessed continuously for 5.5 minutes after stimulus application using a computer-assisted visual analogue scale (COVAS). In both healthy and AD subjects, the mean and peak intensity of itch were higher after the application of cowhage compared to histamine, and were higher after the combined application of cowhage and histamine, compared to histamine alone (p<0.0001 in all cases). Itch intensity ratings were not significantly different between healthy and AD subjects for the same itch inducer used; however AD subjects exhibited a prolonged itch response in comparison to healthy subjects ( p<0.001). Cowhage induced a more intense itch sensation compared to histamine. Cowhage was the dominant factor in itch perception when both pathways were stimulated in the same time. Cowhage-induced itch is a suitable model for the study of itch in AD and other chronic pruritic diseases, and it can serve as a new model for testing antipruritic drugs in humans.
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影响因子：
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作者：
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