The Protective Effect of Selenium on the Chicken Pancreas against Cadmium Toxicity via Alleviating Oxidative Stress and Autophagy
The Protective Effect of Selenium on the Chicken Pancreas against Cadmium Toxicity via Alleviating Oxidative Stress and Autophagy
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DOI：
10.1007/s12011-017-1186-9
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发表时间：
2018-07-01
影响因子：
3.9
通讯作者：
Li, Shu
中科院分区：
文献类型：
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作者：
Liu, Ruohan;Jia, Tiantian;Li, Shu
镉（Cd）是一种剧毒重金属，可影响人类和动物健康。硒（Se）是人体必需的微量元素，可以保护各种器官免受有毒重金属的侵害。虽然许多研究已经研究了Cd对大鼠和其他几种动物的不良影响，但关于Cd诱导鸡胰腺自噬的机制以及硒对Cd的拮抗作用知之甚少。在本研究中，我们给鸡喂硒、Cd或硒和Cd补充剂，建立了硒和Cd相互作用模型，并测量了鸡胰腺中硒和Cd的浓度。观察各组鸡胰腺超微结构变化，并检测各组氧化应激指标。同时检测自噬相关基因的表达水平。结果表明，Cd暴露可使Cd浓度升高，总超氧化物歧化酶（T-SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性升高；鸡胰腺总抗氧化能力（T-AOC）含量。Cd暴露条件下，dynein、Beclin1、LC3-1、LC3-2、Atg5蛋白表达水平升高，TOR蛋白表达水平降低。而硒能明显缓解镉引起的变化。本研究提示Cd可在鸡胰腺中积累，引起氧化应激和自噬。硒通过减少镉积累、减轻氧化应激和抑制自噬来拮抗镉毒性。本研究揭示了硒对镉拮抗的具体机制，可能为镉中毒解毒提供新的线索。
Cadmium (Cd) is a highly toxic heavy metal that can affect human and animal health. Selenium (Se) is an essential microelement that can protect various organs against toxic heavy metals. Although many studies have investigated the adverse effect of Cd in rats and several other animals, little is known regarding the mechanisms of Cd-induced autophagy in the chicken pancreas and the antagonistic effect of Se on Cd. In the current study, we fed chickens Se, Cd, or Se and Cd supplements to establish the Se and Cd interaction model and to measure the concentrations of Se and Cd in the chicken pancreas. The ultrastructure changes of the chicken pancreas were also observed, and we detected oxidative stress indexes in each group. The expression levels of autophagy-related genes were also examined. We found that Cd exposure could increase the concentration of Cd, the activities of total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px); and the total antioxidant capacity (T-AOC) content in the chicken pancreas. The protein expression levels of dynein, Beclin1, LC3-1, LC3-2, and Atg5 were increased and that of TOR was decreased under Cd exposure conditions. However, the changes induced by Cd were significantly alleviated by Se. This study suggested that Cd could accumulate in the chicken pancreas and lead to oxidative stress and autophagy. Se was shown to antagonize Cd toxicity though reducing Cd accumulation, alleviating oxidative stress, and inhibiting autophagy. This study revealed a concrete mechanism for the Se antagonism of Cd and might provide a new clue for the detoxification of Cd poisoning.