Interactions between Bt-expressing tomato and non-target insects: the aphid Macrosiphum euphorbiae and its natural enemies
Interactions between Bt-expressing tomato and non-target insects: the aphid Macrosiphum euphorbiae and its natural enemies
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DOI：
10.1080/17429145.2011.632695
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Guerrieri, Emilio
中科院分区：
文献类型：
--
作者：
Digilio, Maria Cristina;Sasso, Raffaele;Guerrieri, Emilio
苏云金芽孢杆菌柏林毒素在针对害虫方面具有高度的特异性。例如，Cry3Bb对鞘翅目有活性。因此，在可持续农业中使用表达不同Bt毒素的转基因植物的框架下，人们对这些作物对环境和非目标生物的影响越来越感兴趣。在实验室条件下，研究了表达Cry3Bb毒素的bt番茄对大黄蜂（Macrosiphum euphorbiae, Thomas）及其天敌大黄蜂（Macrolophus caliginosus Wagner）和拟内寄生蜂蚜虫（Aphidius ervi Haliday）的影响。在转基因番茄植株（系UC82Bt）上，大黄蜂的表现与近等基因对照（系UC82）无显著差异。免疫分析未检测到Cry3Bb蛋白在表达bt的番茄植株上生长。同样，在UC82Bt和UC82上饲养的蚜虫，对M. caliginosus的寿命和食用量也没有显著差异。此外，基因改造不影响未侵染番茄植株对夜蛾的吸引力。因此，在实验室条件下，表达cry3bb的番茄植株对非目标食草性大戟分枝杆菌及其天敌caliginosus和A. ervi的生物学参数没有表现出任何急性不良影响。
Bacillus thuringiensis Berliner toxins are highly specific in terms of insect pest targeted. For example, Cry3Bb is active against Coleoptera. As a consequence, in the framework of using genetically modified plants expressing different Bt toxins in sustainable agriculture, there is a growing interest about the impact of these crops on the environment and on non-target organisms. The effects of genetically modified Bt-tomato plants expressing the toxin Cry3Bb on the aphid Macrosiphum euphorbiae (Thomas) and its natural enemies, the generalist predator Macrolophus caliginosus Wagner and the endoparasitoid wasp Aphidius ervi Haliday, were assessed under laboratory conditions. No significant differences between performance of M. euphorbiae were observed on genetically modified tomato plants (line UC82Bt) with respect to their near-isogenic control line (line UC82). Immunoassays did not detect Cry3Bb protein in M. euphorbiae developing on Bt-expressing tomato plants. Similarly, no significant differences were found on the longevity and prey consumption of M. caliginosus when fed aphids reared on UC82Bt or on UC82. Moreover, the genetic modification did not affect the attractiveness of uninfested tomato plants toward A. ervi. It is therefore concluded that Cry3Bb-expressing tomato plants did not show any acute adverse effects on the biological parameters of the non-target herbivore M. euphorbiae or its natural enemies, M. caliginosus and A. ervi, under laboratory conditions.