The ISWI chromatin-remodeling protein is required for gene expression and the maintenance of higher order chromatin structure in vivo
The ISWI chromatin-remodeling protein is required for gene expression and the maintenance of higher order chromatin structure in vivo
复制标题
DOI：
10.1016/s1097-2765(00)80430-x
复制
发表时间：
2000-02-01
期刊：
影响因子：
16
通讯作者：
Tamkun, JW
中科院分区：
文献类型：
--
作者：
Deuring, R;Fanti, L;Tamkun, JW
果蝇 ISWI 是 ATP 酶 SW12/SNF2 家族的高度保守成员，是三种染色质重塑复合物：NURF、CHRAC 和 ACF 的催化亚基。为了阐明 ISWI 的生物学功能，我们生成并表征了无效 ISWI 突变和显性失活 ISWI 突变。我们发现 ISWI 突变影响果蝇发育过程中的细胞活力和基因表达。 ISWI 突变还会引起男性 X 染色体结构的显着改变。 ISWI 蛋白不与唾液腺多线染色体上的 RNA Pol II 共定位，表明 ISWI 在转录抑制中可能发挥作用。这些发现揭示了 ISWI ATP 酶的新功能，并强调了其在体内染色质重塑中的重要性。
Drosophila ISWI, a highly conserved member of the SW12/SNF2 family of ATPases, is the catalytic subunit of three chromatin-remodeling complexes: NURF, CHRAC, and ACF. To clarify the biological functions of ISWI, we generated and characterized null and dominant-negative ISWI mutations. We found that ISWI mutations affect both cell viability and gene expression during Drosophila development. ISWI mutations also cause striking alterations in the structure of the male X chromosome. The ISWI protein does not colocalize with RNA Pol II on salivary gland polytene chromosomes, suggesting a possible role for ISWI in transcriptional repression. These findings reveal novel functions for the ISWI ATPase and underscore its importance in chromatin remodeling in vivo.