AN ENHANCER AT THE 3' END OF THE MOUSE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS
AN ENHANCER AT THE 3' END OF THE MOUSE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS
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DOI：
10.1093/nar/19.4.933
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发表时间：
1991-02-25
影响因子：
14.9
通讯作者：
ECKHARDT, LA
中科院分区：
文献类型：
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作者：
LIEBERSON, R;GIANNINI, SL;ECKHARDT, LA
组织特异性增强子（E-mu）位于免疫球蛋白重链（IgH）基因座的连接（J（H））和mu恒定区（C-mu）基因区段之间。由于小鼠内源性IgH基因在其不存在的情况下有效地转录，该增强子的正常功能仍然不明确。最近，另一种同等强度的淋巴特异性增强子已被确定在大鼠IgH基因座的3'端。我们从小鼠中分离到一个类似序列，并将其定位于BALB/c小鼠基因座3 ′端恒定区基因（C-α-membrane）的3 ′端12.5kb。小鼠和大鼠的序列有82%的同源性，并与其他增强子共享几个能够结合蛋白质的DNA序列基序。然而，在瞬时转染测定中，小鼠序列表现为较弱的增强子。讨论了该远端元件在E-mu缺陷、产生免疫球蛋白的细胞系和正常B细胞发育期间内源性IgH基因表达中的作用。
A tissue-specific enhancer (E-mu) lies between the joining (J(H)) and mu constant region (C-mu) gene segments of the immunoglobulin heavy chain (IgH) locus. Since mouse endogenous IgH genes are efficiently transcribed in its absence, the normal function of this enhancer remains ill-defined. Recently, another lymphoid-specific enhancer of equal strength has been identified 3' of the rat IgH locus. We have isolated an analogous sequence from mouse and have mapped it 12.5kb 3' of the 3'-most constant region gene (C-alpha-membrane) of the BALB/c mouse locus. The mouse and rat sequences are 82% homologous and share with other enhancers several DNA sequence motifs capable of binding protein. However, in transient transfection assays, the mouse sequence behaves as a weaker enhancer. The role of this distant element in the expression of endogenous IgH genes, both in E-mu-deficient, Ig-producing cell lines and during normal B cell development, is discussed.