MUTUALLY EXCLUSIVE EXPRESSION OF ALTERNATIVELY SPLICED FMRFAMIDE TRANSCRIPTS IN IDENTIFIED NEURONAL SYSTEMS OF THE SNAIL LYMNAEA
MUTUALLY EXCLUSIVE EXPRESSION OF ALTERNATIVELY SPLICED FMRFAMIDE TRANSCRIPTS IN IDENTIFIED NEURONAL SYSTEMS OF THE SNAIL LYMNAEA
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DOI：
10.1523/jneurosci.13-06-02719.1993
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发表时间：
1993-06-01
影响因子：
5.3
通讯作者：
BENJAMIN, PR
中科院分区：
文献类型：
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作者：
BRIGHT, K;KELLETT, E;BENJAMIN, PR
蜗牛的FMRFamide基因在不同的外显子上编码四肽（FMRFamide/FLRFamide）和七肽（GDPFLRFamide/SDPFLRFamide）。这些外显子的特异性原位杂交探针被用来映射的两个外显子在确定的中枢神经系统的表达。对200多份标本的分析表明，mRNA的胞质表达只有一种类型，单个神经元表达四肽或七肽外显子。在表达两个外显子的约340个神经元中，大多数（80%）表达四肽外显子。四肽外显子分布更广，发生在所有11个神经节的中枢神经系统的神经元。七肽主要局限于两个神经节（内脏和右顶叶），在其他三个神经节中有少量细胞。映射研究结合染料标记的识别神经元显示存在的四肽外显子在几个行为上重要的网络：心脏运动神经元，全身退缩反应运动神经元，可能阴茎运动神经元以及巨大的识别神经元（LP 1，RPD 1）。七肽在两个主要的细胞群（Bgp和Fgp）中是突出的，同时有少量的四肽表达细胞。
The FMRFamide gene of the snail Lymnaea encodes tetrapeptides (FMRFamide/FLRFamide) and heptapeptides (GDPFLRFamide/SDPFLRFamide) on separate exons. In situ hybridization probes specific to these exons were used to map the expression of the two exons in identified neuronal systems of the CNS. Analysis of more than 200 preparations showed that cytoplasmic expression of mRNA was exclusively of one type, with individual neurons expressing either the tetrapeptide or heptapeptide exon. Of the approximately 340 neurons expressing the two exons, the majority (80%) expressed the tetrapeptide exon. The tetrapeptide exon was more wide-spread, occurring in neurons from all 11 ganglia of the CNS. The heptapeptide was mainly confined to two ganglia (visceral and right parietal), with a small number of cells in three other ganglia. Mapping studies combined with dye marking of identified neurons showed the presence of the tetrapeptide exon in several behaviorally important networks: heart motoneurons, whole body withdrawal response motoneurons, and probably penis motoneurons as well as giant identified neurons (LP1, RPD1). The heptapeptides were prominent in two main clusters of cells (Bgp and Fgp) together with a smaller number of tetrapeptide-expressing cells.