SULFATED MUCOPOLYSACCHARIDE SYNTHESIS AND SECRETION IN ENDOTHELIAL CELL-CULTURES
SULFATED MUCOPOLYSACCHARIDE SYNTHESIS AND SECRETION IN ENDOTHELIAL CELL-CULTURES
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DOI：
10.1016/0014-4827(73)90388-1
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发表时间：
1973-01-01
影响因子：
3.7
通讯作者：
BUONASSISI, V
中科院分区：
文献类型：
--
作者：
BUONASSISI, V
兔主动脉管腔内的细胞已获得足够的数量进行大量培养。用~（35）S-硫酸孵育的细胞的放射自显影和从细胞和培养基中提取的~（35）S粘多糖的电泳分析表明，这些细胞合成和分泌各种硫酸化粘多糖。在稳定状态下，细胞内只有一种主要成分和一种次要成分。主要的细胞内成分对软骨素酶ABC的降解具有抗性。在24小时标记期结束时，在培养基中发现两个主要组分和一个次要组分。从培养基中提取的两种主要成分在细胞中的含量都不明显。
The cells that line the lumen of rabbit aorta have been obtained in a quantity sufficient for mass culture. Autoradiography of the cells incubated with35S-sulfuric acid and electrophoretic analysis of35S mucopolysaccharides extracted from the cells and from the medium indicate that these cells synthesize and secrete various species of sulfated mucopolysaccharides. In the steady state, only one major and one minor component are found inside the cell. The major intracellular component is resistant to degradation by chondroitinase ABC. Two major fractions and one minor fraction are found in the culture medium at the end of a 24 h labelling period. Both of the major components extracted from the medium are not present in the cell in an appreciable amount.