Urinary albumin and transferrin as early diagnostic markers of chronic kidney disease.
Urinary albumin and transferrin as early diagnostic markers of chronic kidney disease.
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DOI：
10.1292/jvms.14-0427
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Suzuki J
中科院分区：
文献类型：
--
作者：
Maeda H;Sogawa K;Sakaguchi K;Abe S;Sagizaka W;Mochizuki S;Horie W;Watanabe T;Shibata Y;Satoh M;Sanda A;Nomura F;Suzuki J
在兽医实践中，猫的肾脏疾病越来越受到关注。早期准确地诊断和识别肾功能不全的病理基础是非常重要的，但临床兽医在这方面的报道很少。为探讨尿白蛋白(u-Alb)和转铁蛋白(u-Tf)的检测在早期诊断中的作用，我们对正常猫(IRISI期)和慢性肾病I期猫(CKD)的L尿液标本进行了无创性的尿白蛋白(u-Alb)和转铁蛋白(u-Tf)测定。正常和I期CKD猫的尿白蛋白水平分别为6.0±4.5和11.2±8.4 mg/dl，u-Tf水平分别为0.09±0.42和0.52±0.79 mg/dl。根据ROC曲线分析，u-Alb和u-Tf的敏感性和特异性均高于目前使用的生物标志物--血肌酐水平。U-Alb的敏感性高于u-Tf，而u-Tf的特异性高于u-Alb。通过使用SDS-PAGE的测量，证实了阈值白蛋白水平(20 mg/dl)的有效性。由于尿中u-Tf的漏出先于u-Alb的漏出，因此将u-Tf纳入生化检测可作为早期诊断猫肾脏疾病的IRIS分期的合适指标。
Feline renal diseases are increasingly noted in veterinary practice. It is important to diagnose and identify the pathological basis of renal dysfunction accurately at an early stage, but there are only a few reports on this area in clinical veterinary medicine. We investigated the efficacy of measurement of urinary albumin (u-Alb) and urinary transferrin (u-Tf) for early diagnosis using 5-µl urine samples collected noninvasively by catheterization from normal (IRIS stage I) cats and cats with stage I chronic kidney disease (CKD). The u-Alb levels in normal and stage I CKD cats were 6.0 ± 4.5 and 11.2 ± 8.4 mg/dl, respectively, and the u-Tf levels were 0.09 ± 0.42 and 0.52 ± 0.79 mg/dl, respectively. Based on ROC curve analysis, the sensitivity and specificity of u-Alb and u-Tf were higher than those of the currently used biomarker, the plasma creatinine level. The sensitivity of u-Alb was higher than that of u-Tf, whereas the specificity of u-Tf was higher than that of u-Alb. The validity of the threshold albumin level (20 mg/dl) was confirmed by measurements using SDS-PAGE. Since leakage of u-Tf in urine precedes leakage of u-Alb, inclusion of u-Tf in biochemistry tests may be appropriate for IRIS staging as a diagnostic marker of early diagnosis of renal disorder in cats.