The phosphotransferase system gene ptsH plays an important role in MnSOD production, biofilm formation, swarming motility, and root colonization in Bacillus cereus 905
The phosphotransferase system gene ptsH plays an important role in MnSOD production, biofilm formation, swarming motility, and root colonization in Bacillus cereus 905
复制标题
磷酸转移酶系统基因 ptsH 在蜡样芽孢杆菌 905 的 MnSOD 产生、生物膜形成、群游运动和根部定殖中发挥重要作用
DOI：
10.1016/j.resmic.2018.10.002
复制
发表时间：
2019-03-01
影响因子：
2.6
通讯作者：
Li, Yan
中科院分区：
文献类型：
--
作者：
Gao, Tantan;Ding, Mingzheng;Li, Yan
根际细菌蜡状芽孢杆菌905能够通过在植物根上有效定植来促进植物生长。编码SodA2的含锰超氧化物歧化酶(MnSOD2)对蜡状芽孢杆菌905在小麦根际的生存起着重要作用。然而，调控SodA2表达的基因和蜡状芽孢杆菌905根际定殖的机制还不是很清楚。在这项研究中，我们发现编码磷酸转移酶系统(PTS)的组氨酸磷酸化蛋白(HPR)的ptsH基因的缺失导致MnSOD2表达下降约60%。有证据表明，ptsH显著影响蜡样芽孢杆菌905的抗氧化性、葡萄糖摄取、生物膜的形成和聚集运动。根定植试验表明，AptsH在小麦根部定殖方面存在缺陷，而互补sodA2基因可以部分恢复小麦对非PTS糖阿拉伯糖的利用能力，以及ptsH基因缺失引起的根定殖。综上所述，根据目前的发现，我们认为PtsH通过多种不明确的机制促进蜡状芽孢杆菌905的根定殖，包括PTS和PTS-糖的吸收，上调MnSOD2的产生，促进生物膜的形成和集群运动。(C)2018年巴斯德研究所。爱思唯尔·马森公司出版。版权所有。
The rhizosphere bacterium Bacillus cereus 905 is capable of promoting plant growth through effective colonization on plant roots. The sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for survival of B. cereus 905 in the wheat rhizosphere. However, the genes involved in regulating sodA2 expression and the mechanisms of rhizosphere colonization of B. cereus 905 are not well elucidated. In this study, we found that the deletion of the ptsH gene, which encodes the histidine-phosphorylatable protein (HPr), a component of the phospho transferase system (PTS), causes a decrease of about 60% in the MnSOD2 expression. Evidences indicate that the ptsH dramatically influences resistance to oxidative stress, glucose uptake, as well as biofilm formation and swarming motility of B. cereus 905. Root colonization assay demonstrated that AptsH is defective in colonizing wheat roots, while complementation of the sodA2 gene could partially restore the ability in utilization of arabinose, a non-PTS sugar, and root colonization caused by the loss of the ptsH gene. In toto, based on the current findings, we propose that PtsH contributes to root colonization of B. cereus 905 through multiple indistinct mechanisms, involving PTS and uptake of PTS-sugars, up-regulation of MnSOD2 production, and promotion of biofilm formation and swarming motility. (C) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.