Effect of sodium alginate on mouse ovary vitrification
Effect of sodium alginate on mouse ovary vitrification
复制标题
海藻酸钠对小鼠卵巢玻璃化冷冻的影响
DOI：
10.1016/j.theriogenology.2018.02.006
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发表时间：
2018-06-01
期刊：
影响因子：
2.8
通讯作者：
Dai, Yanfeng
中科院分区：
文献类型：
--
作者：
Liu, Gang;Li, Shubin;Dai, Yanfeng
卵巢组织玻璃化冻融后自体移植的存活率有待提高。因此，寻找一种理想的冷冻保护剂，减少玻璃化冷冻对卵巢的损伤，将为卵巢冷冻技术的临床应用铺平道路。研究了玻璃化冷冻保护液中添加海藻酸钠对小鼠卵巢的影响。从6周龄的CD 1获得的卵巢从A到F被分成6组。A组为正常对照组，不做任何处理。B组为实验对照组，采用含15% Me_2SO和EG的基础冷冻保护液进行冻存。实验组为C、D、E和F组，分别用添加有0.05%、0.10%、0.15%和0.20%海藻酸钠的基础冷冻保护剂溶液冷冻保存。体外分析结果表明，随着冷冻保护液中海藻酸钠浓度的增加，卵母细胞的体外发育能力显著提高（A = 70 +/- 2; B = 43 +/- 2; 48 +/- 3; D = 53 +/- 3; E = 60 +/- 3; B < C < D < A，P
The survival rate of vitrified-thawed ovarian tissues after autotransplantation still needs to be improved. Therefore finding an ideal cryoprectant to reduce the damage to ovaries that caused by vitrification will pave the way for application of ovary cryopreservation on clinics. Experiments were conducted to investigate the effect of sodium alginate in cryoprotectant solution on mouse ovaries during the vitrification process. The ovaries obtained from 6-weeks old CD1 were assigned into six groups from A to F. Group A without treatment was used as the normal control. Group B cryopreserved with the basic cryoprotectant solution containing 15% each Me2SO and EG was used as the experimental control. Groups C, D, E, and F cryopreserved with the basic cryoprotectant solution supplemented with 0.05%, 0.10%, 0.15%, and 0.20% of sodium alginate, respectively, were assigned for the experimental groups. The in vitro analyses showed that the developmental capability of the oocytes isolated from vitrified-thawed ovaries significantly increased with increasing concentration of sodium alginate in the cryoprotectant solution (groups: A = 70 +/- 2; B = 43 +/- 2; 48 +/- 3; D = 53 +/- 3; E = 60 +/- 3; B < C < D < A, P