Sympatric Drosophila simulans flies with distinct mtDNA show difference in mitochondrial respiration and electron transport
Sympatric Drosophila simulans flies with distinct mtDNA show difference in mitochondrial respiration and electron transport
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DOI：
10.1016/j.ibmb.2006.11.002
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发表时间：
2007-03-01
影响因子：
3.8
通讯作者：
Ballard, J. William O.
中科院分区：
文献类型：
--
作者：
Katewa, Subhash D.;Ballard, J. William O.
线粒体DNA （mtDNA）在线粒体代谢中的作用尚未得到充分研究，但携带特定mtDNA类型的人类衰老速度不同，对各种疾病的易感性不同，对各种环境条件的适应也不同。本研究比较了在肯尼亚同栖地收集的具有不同mtDNA单倍群（sill和-III）的拟果蝇雄性的线粒体呼吸、质子泄漏和电子传递。尽管单倍群之间存在很大的差异，但单倍群内变异非常低，核基因组中未发现相关变异。我们发现，携带siII和siIII mtDNA的11龄雄性之间存在可重复的生物能量差异。具有siIII mtDNA的雄性具有更高的(i)从分离的线粒体中获得复合物i和复合物III底物的状态3呼吸速率，以及（ii）复合物IV（细胞色素c氧化酶）活性。携带siIII mtDNA的雄性具有较低的(i)复合物i和III的过氧化氢形成，（ii）分离线粒体的质子泄漏，（III）线粒体atp酶活性，以及（iv）线粒体细胞色素含量。综上所述，结果表明，从含有siIII mtDNA的雄性中分离出的线粒体比含有仍然mtDNA的雄性具有更有效的代谢。(c) 2006 Elsevier Ltd.版权所有。
The role of mitochondrial DNA (mtDNA) in mitochondrial metabolism is understudied yet humans harboring specific mtDNA types age at dissimilar rates, are unequally susceptible to various diseases, and differentially adapt to various environmental conditions. This study compares mitochondrial respiration, proton leak and electron transport of Drosophila simulans males with distinct mtDNA haplogroups (sill and -III) that were collected in sympatry in Kenya. Despite the large divergence among haplogroups there is very low intrahaplogroup variation and no correlated variation in the nuclear genome has been detected. We show that repeatable bioenergetic differences exist between 11d old males harboring siII and siIII mtDNA. Males with siIII mtDNA showed higher (i) state 3 respiration rates from isolated mitochondria for both complex I and complex III based substrates, and (ii) complex IV (cytochrome c oxidase) activity. Males harboring siIII mtDNA had lower (i) hydrogen peroxide formation by both complexes I and III, (ii) proton leak from isolated mitochondria, (iii) mitochondrial ATPase activity, and (iv) mitochondrial cytochrome content. In combination, the results suggest that mitochondria isolated from siIII mtDNA harboring males have more efficient metabolism than sill mtDNA harboring males. (c) 2006 Elsevier Ltd. All rights reserved.