Ultrasound-assisted non-viral gene transfer to the salivary glands
Ultrasound-assisted non-viral gene transfer to the salivary glands
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DOI：
10.1038/gt.2010.86
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发表时间：
2010-11-01
期刊：
影响因子：
5.1
通讯作者：
Benza, R. L.
中科院分区：
文献类型：
--
作者：
Passineau, M. J.;Zourelias, L.;Benza, R. L.
我们报告了一种非病毒基因转移方法，使用超声诱导的微泡破坏，使质粒基因转移载体的小鼠唾液腺细胞的摄取。由巨细胞病毒（CMV）启动子驱动的荧光素酶（Luc）报告基因通过导管后插管单侧递送至下颌下腺，并用体内成像监测Luc表达。将CMV-Luc质粒在含有由脂质包裹的全氟丙烷气体组成的微泡的载体溶液中递送至唾液腺，使用两种不同浓度的微泡（100和15%体积/体积）。使用相同CMV-Luc表达盒的腺病毒（Ad）载体以两种不同剂量用作阳性对照。而超声辅助基因转移（UAGT）与100%的微泡是弱的，并迅速熄灭，UAGT与15%的微泡溶液是强大的和稳定的28天。UAGT似乎是一种可行的和有前途的非病毒基因转移到唾液腺的方法。Gene Therapy（2010）17，1318-1324; doi：10.1038/gt.2010.86; 2010年5月27日在线发表
We report a non-viral gene transfer method using ultrasound induced microbubble destruction to allow the uptake of plasmid gene transfer vectors to the cells of the mouse salivary gland. The Luciferase (Luc) reporter gene, driven by a cytomegalovirus (CMV) promoter, was delivered unilaterally to the submandibular salivary gland via retroductal cannulation and Luc expression was monitored with in vivo imaging. The CMV-Luc plasmid was delivered to the salivary gland in a carrier solution containing microbubbles composed of lipid-encased perfluoropropane gas, with two different concentrations of microbubbles used (100 and 15% volume/volume). An Adenoviral (Ad) vector using an identical CMV-Luc expression cassette was used as a positive control at two different dosages. Whereas ultrasound-assisted gene transfer (UAGT) with 100% microbubbles was weak and rapidly extinguished, UAGT with the 15% microbubble solution was robust and stable for 28 days. UAGT seems to be a practicable and promising method for non-viral gene delivery to the salivary glands. Gene Therapy (2010) 17, 1318-1324; doi:10.1038/gt.2010.86; published online 27 May 2010