Effects of single-prolonged stress on neurons and their afferent inputs in the amygdala
Effects of single-prolonged stress on neurons and their afferent inputs in the amygdala
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DOI：
10.1016/j.neuroscience.2007.12.028
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发表时间：
2008-03-27
期刊：
影响因子：
3.3
通讯作者：
Kawata, M.
中科院分区：
文献类型：
--
作者：
Cui, H.;Sakamoto, H.;Kawata, M.
杏仁核通过存储与情感相关的信息来调节记忆巩固，并在恐惧和焦虑中发挥关键作用。我们研究了神经元形态和神经递质含量的变化，在大鼠杏仁核暴露于一个单一的长期压力（SPS）作为一个假定的动物模型，人类创伤后应激障碍（PTSD）。大鼠灌注7天后SPS，和细胞内注射的荧光黄管理的神经元的基底外侧（BLA）和中央杏仁核（CeA），以分析在细胞水平上的形态学变化。BLA锥体神经元的树突分支显著增加，但对CeA神经元无影响。正常情况下，BLA中富含神经肽Y（NPY）。与对照组相比，SPS暴露组大鼠BLA内NPY免疫反应阳性纤维的局部浓度和数量增加。在CeA中未观察到这方面的差异。荧光和电子显微镜双重免疫染色显示，NPY免疫反应阳性终末与BLA中的钙/钙调素H依赖性蛋白激酶（CaMKII：锥体神经元的标记物）阳性神经元密切相关，BLA中的神经元对糖皮质激素受体（GR）和盐皮质激素受体（MR）呈免疫阳性。SPS对BLA中CaMK Ⅱ和MR/GR的表达无明显影响。基于这些研究结果，我们认为，在BLA的锥体神经元的形态学的变化，SPS可以介导通过增强神经肽Y的功能，这种结构可塑性在杏仁核提供了一个细胞和分子基础，以了解情感障碍。(C)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The amygdala modulates memory consolidation with the storage of emotionally relevant information and plays a critical role in fear and anxiety. We examined changes in neuronal morphology and neurotransmitter content in the amygdala of rats exposed to a single prolonged stress (SPS) as a putative animal model for human post-traumatic stress disorder (PTSD). Rats were perfused 7 days after SPS, and intracellular injections of Lucifer Yellow were administered to neurons of the basolateral (BLA) and central amygdala (CeA) to analyze morphological changes at the cellular level. A significant increase of dendritic arborization in BLA pyramidal neurons was observed, but there was no effect on CeA neurons. Neuropeptide Y (NPY) was abundant in BLA under normal conditions. The local concentration and number of immunoreactive fibers of NPY in the BLA of SPS-exposed rats were increased compared with the control. No differences were observed in this regard in the CeA. Double immunostaining by fluorescence and electron microscopy revealed that NPY immunoreactive terminals were closely associated with calcium/calmodulin H-dependent protein kinase (CaMKII: a marker for pyramidal neurons)-positive neurons in the BLA, which were immunopositive to glucocorticoid receptor (GR) and mineralocorticoid receptor (MR). SPS had no significant effect on the expression of CaMKII and MR/GR expression in the BLA. Based on these findings, we suggest that changes in the morphology of pyramidal neurons in the BLA by SPS could be mediated through the enhancement of NPY functions, and this structural plasticity in the amygdala provides a cellular and molecular basis to understand for affective disorders. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.