REACH: Robotic Equipment for Automated Crystal Harvesting using a six-axis robot arm and a micro-gripper.
REACH: Robotic Equipment for Automated Crystal Harvesting using a six-axis robot arm and a micro-gripper.
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REACH：使用六轴机械臂和微型夹具进行自动晶体收获的机器人设备。
DOI：
10.1107/s0907444912048019
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
J. Ferrer
中科院分区：
文献类型：
--
作者：
Mohammad Yaser Heidari Khajepour;X. Vernède;D. Cobessi;H. Lebrette;Pierrick Rogues;M. Terrien;C. Berzin;J. Ferrer
在蛋白质晶体学实验中，只有两个关键步骤仍然是手工操作的：将晶体从最初的结晶液滴转移到低温保护溶液中，然后快速冷却。这些步骤既危险又繁琐，需要高度的手工灵巧性。这些限制性步骤是高通量晶体学的真实的瓶颈，并限制了蛋白质晶体学核心设施的远程使用。为了消除这种限制，开发了自动晶体收获机器人设备（REACH）。这种自动化系统配备了双指微夹持装置，可以进行晶体收获、低温保护和快速冷却。使用这种设置，收获实验进行了几个晶体，然后直接数据收集使用相同的机械臂作为测角仪。衍射数据的分析表明，REACH是高度可靠和有效的，不会改变晶体学数据。这款新仪器填补了高通量晶体学管道中的差距。
In protein crystallography experiments, only two critical steps remain manual: the transfer of crystals from their original crystallization drop into the cryoprotection solution followed by flash-cooling. These steps are risky and tedious, requiring a high degree of manual dexterity. These limiting steps are a real bottleneck to high-throughput crystallography and limit the remote use of protein crystallography core facilities. To eliminate this limit, the Robotic Equipment for Automated Crystal Harvesting (REACH) was developed. This robotized system, equipped with a two-finger micro-gripping device, allows crystal harvesting, cryoprotection and flash-cooling. Using this setup, harvesting experiments were performed on several crystals, followed by direct data collection using the same robot arm as a goniometer. Analysis of the diffraction data demonstrates that REACH is highly reliable and efficient and does not alter crystallographic data. This new instrument fills the gap in the high-throughput crystallographic pipeline.
用于自动确定大分子结构的Phenix软件。
影响因子：
4.8
作者：
Adams, Paul D.;Afonine, Pavel V.;Bunkoczi, Gabor;Chen, Vincent B.;Echols, Nathaniel;Headd, Jeffrey J.;Hung, Li-Wei;Jain, Swati;Kapral, Gary J.;Kunstleve, Ralf W. Grosse;McCoy, Airlie J.;Moriarty, Nigel W.;Oeffner, Robert D.;Read, Randy J.;Richardson, David C.;Richardson, Jane S.;Terwilliger, Thomas C.;Zwart, Peter H.
通讯作者：
Zwart, Peter H.
使用微机械硅工具进行自动划线播种。
DOI：
10.1107/s0907444906023705
发表时间：
2006
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Georgiev,Atanas;Vorobiev,Sergey;Edstrom,William;Song,Ting;Laine,Andrew;Hunt,John;Allen,Peter
通讯作者：
Allen,Peter