Evidence for an inflammation-induced change in the local glutamatergic regulation of postganglionic sympathetic efferents
Evidence for an inflammation-induced change in the local glutamatergic regulation of postganglionic sympathetic efferents
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DOI：
10.1016/s0304-3959(99)00098-6
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发表时间：
1999-11-01
期刊：
影响因子：
7.4
通讯作者：
Carlton, SM
中科院分区：
文献类型：
--
作者：
Coggeshall, RE;Carlton, SM
交感神经传出与炎症引起的疼痛有关。因此对这些纤维的控制是一个相当重要的问题，在这方面，正常大鼠节后交感神经纤维表达嗜离子性谷氨酸受体。目前的研究验证了炎症导致表达这些受体的交感神经传出信号数量显著增加的假设。在正常大鼠中，L4和L5交感灰色支中纤维免疫染色的抗体分别针对NMDA (NMDARI), AMPA （GluR1）或kainate （glur5,6,7）受体亚基，分别为29.5%，5%和5%。在一只后爪注射完全弗氏佐剂48小时后，同侧灰色支纤维免疫染色NMDA、AMPA和kainate的百分比分别为57、52和48%。因此，炎症后，表达NMDA受体的轴突增加了两倍，表达AMPA或盐酸盐受体的轴突增加了十倍。这些数据表明，炎症期间谷氨酸受体的激活可能会增强神经节后活性。神经节后纤维活性的增加可能导致神经节后神经递质和其他物质（如前列腺素）的释放增加，从而增强伤害感受器活性。谷氨酸受体组织的这种变化为外周炎症后经常出现的适应不良症状提供了可能的药物干预位点。(C) 1999年国际疼痛研究协会。Elsevier Science B.V.出版
Sympathetic efferents are involved in the pain of inflammation. Thus the control of these fibers is a matter of considerable importance, in this regard, postganglionic sympathetic fibers in normal rats express ionotropic glutamate receptors. The present study tests the hypothesis that inflammation leads to a significant increase in numbers of sympathetic efferents that express these receptors.;In normal rats, the percentage of fibers in the L4 and L5 sympathetic gray rami immunostained with antibodies against subunits of NMDA (NMDARI), AMPA (GluR1), or kainate (GluR5,6,7) receptors are 29, 5 and 5%, respectively. Forty-eight hours following injection of complete Freund's adjuvant into one hindpaw, the percentages of fibers in the ipsilateral gray rami immunostained for NMDA, AMPA or kainate are 57, 52 and 48% respectively. Thus, following inflammation there is a two-fold increase in axons expressing NMDA receptors and a ten-fold increase in axons expressing AMPA or kainate receptors. These data suggest that postganglionic activity may be enhanced by glutamate receptor activation during inflammation. Increased activity in postganglionic fibers could lead to an increased release of NE and other substances in postganglionic efferents such as prostaglandins which in turn could enhance nociceptor activity. This change in glutamate receptor organization offers a possible site of pharmacological intervention for the maladaptive symptoms that often arise following peripheral inflammation. (C) 1999 International Association for the Study of Pain. Published by Elsevier Science B.V.