In vitro activities of daptomycin, vancomycin, quinupristin-dalfopristin, linezolid, and five other antimicrobials against 307 gram-positive anaerobic and 31 Corynebacterium clinical isolates
In vitro activities of daptomycin, vancomycin, quinupristin-dalfopristin, linezolid, and five other antimicrobials against 307 gram-positive anaerobic and 31 Corynebacterium clinical isolates
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DOI：
10.1128/aac.47.1.337-341.2003
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发表时间：
2003-01-01
影响因子：
4.9
通讯作者：
Fernandez, HT
中科院分区：
文献类型：
--
作者：
Goldstein, EJC;Citron, DM;Fernandez, HT
通过NCCLS参考琼脂稀释法测定了达托霉素（一种环脂肽）和其他8种药物对338株革兰氏阳性厌氧菌和棒状杆菌的活性，其中厌氧菌用补充布鲁氏菌琼脂，棒状杆菌用Mueller-Hinton琼脂。在补充Ca 2+（50 mg/L）的布鲁氏菌琼脂平板上测定的达托霉素MIC比在未补充培养基中测定的低1 - 4倍。达托霉素对许多菌株具有高活性（MIC，小于或等于2 μ g/ml），包括37种消化链球菌中的36种，真杆菌属48种分离株中的37种，以及丙酸杆菌属的所有菌株，产气荚膜梭菌、艰难梭菌和其他梭菌属。它对无害梭菌和34株万古霉素耐药乳杆菌中的16株的活性是万古霉素的4倍或更高。3株C.喹奴普汀-达福普汀和利奈唑胺MIC> 8 μ g/ml的艰难梭菌被< 1 μ g/ml的达托霉素抑制。大多数梭状芽孢杆菌、副腐殖梭菌、第三梭菌和多枝梭菌菌株的达托霉素MIC大于或等于4 μ g/ml;分离株通常对其他抗菌剂更具耐药性。达托霉素对放线菌的活性低2 - 4倍。比万古霉素、奎奴普汀-达福普汀或利奈唑胺更有效。棒状杆菌属的31个菌株中的29个，包括杰氏棒状杆菌、无菌棒状杆菌和假白喉棒状杆菌，被小于或等于0.25 μ g/ml的达托霉素抑制。对于两种“水生棒状杆菌”菌株，需要8 μ g/ml达托霉素进行抑制。达托霉素表现出对广泛的革兰氏阳性菌包括耐万古霉素的C。innocuum和peptide菌株以及奎奴普汀-达福普汀-和利奈唑胺耐药C.艰难菌株
The activities of daptomycin, a cyclic lipopeptide, and eight other agents were determined against 338 strains of gram-positive anaerobic bacteria and corynebacteria by the NCCLS reference agar dilution method with supplemented brucella agar for the anaerobes and Mueller-Hinton agar for the corynebacteria. The daptomycin MICs determined on Ca2+-supplemented (50 mg/liter) brucella agar plates were one- to fourfold lower than those determined in unsupplemented media. Daptomycin was highly active (MICs, less than or equal to2 mug/ml) against many strains including 36 of 37 peptostreptococci, 37 of 48 isolates of the Eubacterium group, and all strains of Propionibacterium spp., Clostridium perfringens, Clostridium difficile, and other Clostridium spp. It was fourfold or greater more active than vancomycin against Clostridium innocuum and 16 of 34 strains of vancomycin-resistant lactobacilli. Three strains of C. difficile for which quinupristin-dalfopristin and linezolid MICs were > 8 mug/ml were inhibited by < 1 mug of daptomycin per ml. Daptomycin MICs were greater than or equal to4 mug/ml for most strains of Clostridium clostridioforme, Clostridium paraputrificum, Clostridium tertium, and Clostridium ramosum; the isolates were generally more resistant to other antimicrobials. Daptomycin was two- to fourfold less active against Actinomyces spp. than vancomycin, quinupristin-dalfopristin, or linezolid. Twenty-nine of 31 strains of Corynebacterium spp., including Corynebacterium jeikeium, Corynebacterium amycolatum, and Corynebacterium pseudodiphtheriticum, were inhibited by less than or equal to0.25 mug of daptomycin per ml. For two strains of "Corynebacterium aquaticum," 8 mug of daptomycin per ml was required for inhibition.. Daptomycin demonstrated very good activities against a broad range of gram-positive organisms including vancomycin-resistant C. innocuum and lactobacillus strains and quinupristin-dalfopristin- and linezolid-resistant C. difficile strains.