A CA-dinucleotide polymorphism at the D17S113 locus, which is closely linked to D17S74.
A CA-dinucleotide polymorphism at the D17S113 locus, which is closely linked to D17S74.
复制标题
D17S113 基因座上存在 CA-二核苷酸多态性，与 D17S74 紧密连锁。
DOI：
10.1093/nar/20.4.923-a
复制
发表时间：
1992
影响因子：
14.9
通讯作者：
Fain,PR
中科院分区：
文献类型：
--
作者：
Barker,DF;Nguyen,K;Fain,PR
染色体定位：通过对一组人/中国仓鼠体细胞杂交和原位杂交进行Southern blot杂交，定位到染色体6q25-q27(2)。孟德尔遗传：共显性分离在6个两代家庭中得到证实。其他评论：扩增反应条件如下：样品经过35个温度循环处理，包括94℃1 min， 55℃1 min和72℃45秒。通过与人Man-6-P/IGF2受体cDNA克隆(1)和M13mpl8 DNA测序阶梯扩增产物的大小进行比较，确定等位基因的大小。致谢：我们感谢WS Sly博士克隆了人Man-6-P/IGF2受体cDNA。JG得到了人类前沿科学计划组织的奖学金支持。GAR得到加拿大医学研究理事会和魁北克卫生研究基金会的支持。这项工作得到了加拿大肌肉协会的支持。参考文献：1)王志强等（1988）。化学，26,2553-2562。2) Laureys， G. et al.(1988) Genomics 3,224 -229。
Chromosomal Localization: Mapped to chromosome 6q25-q27 by Southern blot hybridization to a panel of human/Chinese hamster somatic cell hybrids and in situ hybridization (2). Mendelian Inheritance: Codominant segregation was demonstrated in six two generation families. Other Comments: Conditions for the amplification reactions were as follows. Samples were processed through 35 temprature cycles consisting of1 min at 94 C, 1 min at 55 C and 45 sec at 72 C. Sizes of the alleles were determined by comparison to the size of the amplified products of human Man-6-P/IGF2 Receptor cDNA clone (1) and M13mpl8 DNA sequencing ladders. Acknowledgements: We thank Dr WS Sly for the human Man-6-P/IGF2 Receptor cDNA clone. JG is supported by a fellowship from Human Frontier Science Program Organization. GAR is supported by the Medical Research Council of Canada and the Fonds de recherche en sante du Qu6bec. This work was supported by the Muscular Association of Canada. References: 1) Oshima, A. et al.(1988) J. Biol. Chem. 263, 2553-2562. 2) Laureys, G. et al.(1988) Genomics 3, 224-229.