Influence of porous tantalum scaffold pore size on osteogenesis and osteointegration: A comprehensive study based on 3D-printing technology
Influence of porous tantalum scaffold pore size on osteogenesis and osteointegration: A comprehensive study based on 3D-printing technology
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DOI：
10.1016/j.msec.2021.112382
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发表时间：
2021-08-26
影响因子：
7.9
通讯作者：
Liao, Junyi
中科院分区：
文献类型：
--
作者：
Luo, Changqi;Wang, Claire;Liao, Junyi
多孔钽（Ta）支架由于其优异的生物学性能在骨组织工程中的应用日益受到关注。然而，孔径和孔隙率哪种更有利于骨缺损修复仍存在争议。本研究采用计算机辅助设计和3D打印技术，构建了孔径分别为100-200、200-400、400-600和600-800 μ m，孔隙率分别为25%、55%、75%和85%的多孔钽支架，并进行了体内外研究。我们发现孔径为400-600 μ m的钽支架在体外表现出更强的促进细胞粘附、增殖和成骨分化的能力。体内实验表明，孔径为400-600 μ m的多孔钽支架具有更好的骨长入和整合性能。在机理上，计算流体动力学分析证明孔径为400-600 μ m的多孔钽支架具有适当的渗透性和表面积，有利于细胞的粘附和增殖。我们的研究结果强烈表明，孔径和孔隙率是必要的多孔钽支架的进一步应用，和多孔钽支架与孔径400-600 μ m有利于骨生成和骨整合。这些发现为多孔钽支架在骨缺损修复中的进一步应用提供了新的证据。
The emerging role of porous tantalum (Ta) scaffold for bone tissue engineering is noticed due to its outstanding biological properties. However, it is controversial which pore size and porosity are more conducive for bone defect repair. In the present work, porous tantalum scaffolds with pore sizes of 100-200, 200-400, 400-600 and 600-800 mu m and corresponding porosities of 25%, 55%, 75%, and 85% were constructed, using computer aided design and 3D printing technologies, then comprehensively studied by in vitro and in vivo studies. We found that Ta scaffold with pore size of 400-600 mu m showed stronger ability in facilitating cell adhesion, proliferation, and osteogenic differentiation in vitro. In vivo tests identified that porous tantalum scaffolds with pore size of 400-600 mu m showed better performance of bone ingrowth and integration. In mechanism, computational fluid dynamics analysis proved porous tantalum scaffolds with pore size of 400-600 mu m hold appropriate permeability and surface area, which facilitated cell adhesion and proliferation. Our results strongly indicate that pore size and porosity are essential for further applications of porous tantalum scaffolds, and porous tantalum scaffolds with pore size 400-600 mu m are conducive to osteogenesis and osseointegration. These findings provide new evidence for further application of porous tantalum scaffolds for bone defect repair.