Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells.
Phenotypic heterogeneity and cytotoxic activity of Con A and IL-2-stimulated cultures of mouse Thy-1+ epidermal cells.
复制标题
Con A 和 IL-2 刺激的小鼠 Thy-1 表皮细胞培养物的表型异质性和细胞毒活性。
DOI：
10.1111/1523-1747.ep12463290
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Tigelaar,RE
中科院分区：
文献类型：
--
作者：
Nixon-Fulton,JL;HackettJr,J;Bergstresser,PR;Kumar,V;Tigelaar,RE
建立了小鼠Thy-1+表皮细胞(EC)的短期和长期培养方法，以研究其表型和功能特性。刀豆蛋白A(ConA)和白介素2(IL-2)刺激Thy-1+EC介导的针对NK敏感靶点YAC-1对NK耐药靶点P815的非MHC导向的细胞毒作用；这些细胞还介导抗体依赖的细胞介导的细胞毒作用(ADCC)，表明至少部分细胞上存在Ig G-FCR。新鲜分离的Thy-1+EC不能裂解YAC-1靶点，但在加入ConA和IL-2培养9d后观察到这种活性。在体内，树突状Thy-1+EC不表达T细胞标志，而L3T4和Lyt-2短期培养的细胞表现出表型的异质性，有少量但显著的Lyt-2+和L3T4+细胞出现。用流式细胞术将短期培养的内皮细胞分为阳性和阴性两组，确定介导细胞毒活性的效应细胞(S)的表型。表达L3T4或缺乏asialo GM1的细胞不裂解YAC-1靶标；在asialo GM1+，Lyt-2-和asialo GM1+，Lyt-2+的细胞群体中发现最大的细胞毒活性。这些研究表明，在ConA和IL-2存在下，小鼠表皮来源的Thy-1+细胞具有产生表型异质性群体的能力，其中一些细胞能够介导细胞毒活性。
Short-term and long-term culture of mouse Thy-1+ epidermal cells (EC) were established in order to characterize their phenotypic and functional properties. Concanavalin A (Con A) and Interleukin 2 (IL-2) stimulated Thy-1+ EC mediated non-MHC directed cytotoxicity preferentially against the NK-sensitive target, YAC-1 vs the NK-resistant target, P815; these cells also mediated antibody-dependent cell-mediated cytotoxicity (ADCC), indicating the presence of IgG-FcR on at least some of them. Freshly isolated Thy-1+ EC failed to lyse YAC-1 targets; however, this activity was observed after 9 d of culture with Con A and IL-2. While dendritic Thy-1+EC, in vivo, do not express the T-cell markers, L3T4 and Lyt-2 short-term cultured cells displayed phenotypic heterogeneity with small but significant percentages of Lyt-2+ and L3T4+ cells appearing transiently. The phenotype of the effect or cell(s), which mediates cytotoxic activity, was determined by utilizing flow cytometery to sort short-term cultured EC into positively and negatively stained populations. Cells which express L3T4, or which lack asialo GM1, did not lyse YAC-1 targets; maximum cytotoxic activity was found within populations of cells which are asialo GM1+, Lyt-2-, and asialo GM1+, Lyt-2+. These studies indicate that Thy-1+ cells derived from mouse epidermis when cultured in the presence of Con A and IL-2 have the capacity to generate a phenotypically heterogeneous population, some cells of which are capable of mediating cytotoxic activities.