Relationship between serum adiponectin concentration, body condition score, and peripheral tissue insulin response of dairy cows during the dry period
Relationship between serum adiponectin concentration, body condition score, and peripheral tissue insulin response of dairy cows during the dry period
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DOI：
10.1016/j.domaniend.2016.12.004
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发表时间：
2017-04-01
影响因子：
2.1
通讯作者：
Opsomer, G.
中科院分区：
文献类型：
--
作者：
De Koster, J.;Urh, C.;Opsomer, G.
本研究旨在探讨干期可变体况评分（BCS）奶牛血清脂联素浓度与外周血组织胰岛素反应的关系。根据BCS (BCS 3.75, n = 5)，在干期初期选择奶牛。从干期开始，每周采血，评估BCS和背膘厚度。每周血液样本分析脂联素浓度使用牛特异性ELISA。在干燥期结束时进行高胰岛素正糖钳试验，以测量外周组织胰岛素反应。建立葡萄糖和脂肪酸代谢的胰岛素剂量反应曲线。回归分析显示，干末期血清脂联素浓度下降（P < 0.05），且与BCS呈负相关（P < 0.05）。在糖代谢水平，血清脂联素浓度与胰岛素反应性呈正相关（反映胰岛素的最大效果，r = 0.76, P < 0.05），与胰岛素敏感性不相关（反映达到半最大效果所需的胰岛素浓度，r = -0.54, P = 0.13）。在脂肪酸代谢水平上，高脂联素浓度与HEC试验中NEFA水平较低呈负相关（r = -0.61, P = 0.08），反映NEFA代谢的胰岛素反应性（r = -0.16, P = 0.67），而与NEFA代谢的胰岛素敏感性无相关性（r = -0.16, P = 0.67）。综上所述，奶牛干期血清脂联素浓度与BCS呈负相关，与葡萄糖和脂肪酸代谢的胰岛素反应性呈正相关。(C) 2016 Elsevier Inc.版权所有。
The aim of the present study was to describe the relationship between serum adiponectin concentration and peripheral tissue insulin response in dairy cows with a variable body condition score (BCS) during the dry period. Cows were selected at the beginning of the dry period based on BCS (BCS 3.75, n = 5). Animals were followed from the beginning of the dry period by weekly blood sampling and assessment of BCS and backfat thickness. Weekly blood samples were analyzed for adiponectin concentration using a bovine specific ELISA. Hyperinsulinemic euglycemic clamp tests were performed at the end of the dry period to measure peripheral tissue insulin response. Insulin dose response curves were established for both glucose and fatty acid metabolism. Regression analysis revealed that the serum concentrations of adiponectin dropped at the end of the dry period (P < 0.05) and were negatively associated with BCS (P < 0.05). At the level of the glucose metabolism, serum concentrations of adiponectin were positively correlated with insulin responsiveness (reflecting the maximal effect of insulin; r = 0.76, P < 0.05), but not with insulin sensitivity (reflecting the insulin concentration needed to achieve halfmaximal effect; r = -0.54, P = 0.13). At the level of the fatty acid metabolism, greater adiponectin concentrations were negatively correlated with lower NEFA levels during the HEC test reflecting the insulin responsiveness of the NEFA metabolism (r = -0.61, P = 0.08), whereas there was no association with the insulin sensitivity of the NEFA metabolism (r = -0.16, P = 0.67). In conclusion, serum concentrations of adiponectin were negatively associated with the BCS of dairy cows during the dry period and positively associated with insulin responsiveness of the glucose and fatty acid metabolism. (C) 2016 Elsevier Inc. All rights reserved.