Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae
Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae
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DOI：
10.1128/aem.01148-06
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发表时间：
2006-12-01
影响因子：
4.4
通讯作者：
Mimuro, Mamoru
中科院分区：
文献类型：
--
作者：
Ohkubo, Satoshi;Miyashita, Hideaki;Mimuro, Mamoru
建立了一种应用PCR-变性梯度凝胶电泳技术检测大型海藻附生植物群落的分子生物学方法。选择性扩增来自蓝细菌或藻质的16 S rRNA基因片段可以提高对小型真菌的检测。Acaryochloris是一种含叶绿素d的蓝藻，在红藻、绿色藻和褐藻中均发现了两种不同类型的Acaryochloris。
A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.