Heterotopic Cervical Heart Transplantation in Mice
Heterotopic Cervical Heart Transplantation in Mice
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DOI：
10.3791/52907
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发表时间：
2015-08-01
影响因子：
1.2
通讯作者：
Krane, Markus
中科院分区：
文献类型：
--
作者：
Ratschiller, Thomas;Deutsch, Marcus-Andre;Krane, Markus
小鼠异位颈部心脏移植是移植和心血管研究的重要工具。袖带技术通过避免小血管的挑战性缝合，从而减少热缺血时间，大大简化了该模型。与腹部移植物植入相比，颈部模型的侵入性较小，植入的移植物易于进行进一步的随访检查。通过将移植物的升主动脉与受体的颈总动脉一起拉过袖带，并通过将主肺动脉与颈外静脉一起拉过袖带来进行吻合。选择合适的袖带尺寸和血管的完全活动对于成功的血运重建是重要的。缺血再灌注（I/R）损伤可以通过灌注心脏停搏液和低温来最小化。在本文中，我们提供了简化和改进的袖套技术的技术细节，这应该允许具有基本显微外科技能的外科医生以高成功率执行该手术。
The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies this model by avoiding challenging suture anastomoses of small vessels thereby reducing warm ischemia time. In comparison to abdominal graft implantation the cervical model is less invasive and the implanted graft is easily accessible for further follow-up examinations. Anastomoses are performed by pulling the ascending aorta of the graft over the cuff with the recipient's common carotid artery and by pulling the main pulmonary artery over the cuff with the external jugular vein. Selection of appropriate cuff size and complete mobilization of the vessels are important for successful revascularization. Ischemia-reperfusion (I/R) injury can be minimized by perfusing the graft with a cardioplegic solution and by hypothermia. In this article, we provide technical details for a simplified and improved cuff technique, which should allow surgeons with basic microsurgical skills to perform the procedure with a high success rate.