A physiological role for neuropeptide Y in regulating the estrogen/progesterone induced luteinizing hormone surge in ovariectomized rats.
A physiological role for neuropeptide Y in regulating the estrogen/progesterone induced luteinizing hormone surge in ovariectomized rats.
复制标题
神经肽 Y 在调节卵巢切除大鼠中雌激素/孕激素诱导的黄体生成素激增中的生理作用。
DOI：
10.1159/000125188
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发表时间：
1989
影响因子：
4.1
通讯作者：
Gaillard,RC
中科院分区：
文献类型：
--
作者：
Wehrenberg,WB;Corder,R;Gaillard,RC
为了验证神经肽Y（NPY）参与调节雌激素/孕酮诱导的促黄体生成激素（LH）激增的假设，我们通过直接向中枢神经系统给予纯化的免疫球蛋白来被动免疫动物。用脑室内（i. c. v.）引导插管和静脉内导管。在实验第1天，动物接受5 µl抗NPY高特异性免疫球蛋白（NPY-ab）i. c. v.注射和50 µg苯甲酸雌二醇注射。在实验的第2天和第3天重复抗体注射。在第3天，动物接受2.5 mg黄体酮注射。对照动物以完全相同的方式处理，不同之处在于i. c. v.注射非特异性对照免疫球蛋白而不是NPY-ab。正如预期的那样，用对照抗体处理的类固醇致敏动物在孕酮注射后约4小时表现出LH分泌的大幅激增。浓度从4.2 ± 1.0纳克/毫升上升到27.9 ± 9.9纳克/毫升。与此形成鲜明对比的是，NPY-ab处理的动物显示LH浓度没有增加。基线值为3.1 ± 0.3 ng/ml，注射孕酮后保持不变（最大浓度为3.8 ± 1.9 ng/ml）。这些结果表明，下丘脑神经肽Y在介导雌二醇/孕酮诱导的促性腺激素激增中起作用，并表明这种神经肽在LH和FSH的正常排卵激增中起生理作用。
To test the hypothesis that neuropeptide Y(NPY) is involved in the regulation of the estrogen/progesterone-induced luteinizing hormone (LH) surge in ovariectomized rats, we passively immunized animals against NPY by administering purified immunoglobulins raised against the peptide directly into the central nervous system. Ovariectomized rats were prepared with an intracerebroventricular (i.c.v.) guide cannula and an intravenous catheter prior to experimentation. On day 1 of the experiment the animals received a 5-µl i.c.v. injection of a highly specific immunoglobulin against NPY(NPY-ab) and a 50-µg injection of estradiol benzoate. The antibody injection was repeated on days 2 and 3 of the experiment. On day 3, animals received a 2.5-mg injection of progesterone. Control animals were treated in exactly the same fashion except that a nonspecific control immunoglobulin was injected i.c.v. rather than the NPY-ab. As expected, the steroid-primed animals treated with the control antibodies exhibited large surges in LH secretion approximately 4 h following the progesterone injection. Concentrations rose from 4.2 ± 1.0 to 27.9 ± 9.9 ng/ml. In marked contrast, the NPY-ab-treated animals demonstrated no increase in LH concentrations. Baseline values were 3.1 ± 0.3 ng/ml and remained unchanged (maximum concentrations were 3.8 ± 1.9 ng/ml) following the progesterone injection. These results demonstrate that hypothalamic NPY plays a role in mediating the estradiol/progesterone-induced gonadotropin surge and suggests that this neuropeptide plays a physiological role in normal ovulatory surges of LH and FSH.