iDNA4mC: identifying DNA N4-methylcytosine sites based on nucleotide chemical properties
iDNA4mC: identifying DNA N4-methylcytosine sites based on nucleotide chemical properties
复制标题
DOI：
10.1093/bioinformatics/btx479
复制
发表时间：
2017-11-15
期刊：
影响因子：
5.8
通讯作者：
Lin, Hao
中科院分区：
文献类型：
--
作者：
Chen, Wei;Yang, Hui;Lin, Hao
DNA N-4-甲基胞嘧啶(4mC)是一种表观遗传修饰。了解4mC的分布有助于了解其生物学功能。虽然已经提出了检测4mC位点的实验方法，但它们对于执行全基因组检测来说代价高昂。因此，有必要开发预测4mC位点的计算方法。在本工作中，我们开发了第一个识别4mC位点的网络服务器iDNA4mC，其中DNA序列编码具有核苷酸化学性质和核苷酸频率。严格的刀切试验和跨物种试验的预测结果表明，iDNA4mC的性能相当有希望，并具有成为鉴定4mC位点的有用工具的潜力。
DNA N-4-methylcytosine (4mC) is an epigenetic modification. The knowledge about the distribution of 4mC is helpful for understanding its biological functions. Although experimental methods have been proposed to detect 4mC sites, they are expensive for performing genome-wide detections. Thus, it is necessary to develop computational methods for predicting 4mC sites.In this work, we developed iDNA4mC, the first webserver to identify 4mC sites, in which DNA sequences are encoded with both nucleotide chemical properties and nucleotide frequency. The predictive results of the rigorous jackknife test and cross species test demonstrated that the performance of iDNA4mC is quite promising and holds high potential to become a useful tool for identifying 4mC sites.