Effects of sex hormones on regulation of ABCG2 expression in the placental cell line BeWo.
Effects of sex hormones on regulation of ABCG2 expression in the placental cell line BeWo.
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发表时间：
2006
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通讯作者：
S. Yasuda;S. Itagaki;T. Hirano;K. Iseki
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作者：
S. Yasuda;S. Itagaki;T. Hirano;K. Iseki
目的探讨妊娠期胎盘分泌的性激素对胎盘细胞系BeWo中ABCG 2 mRNA和蛋白表达水平的影响。方法采用逆转录-聚合酶链反应（RT-PCR）方法研究雌激素（雌酮、17-β-雌二醇和雌三醇）对ABCG 2 mRNA表达水平的影响。Western blot分析ABCG 2蛋白的表达水平。我们还研究了定位的ABCG 2在BeWo细胞的质膜组分的蛋白质印迹分析和免疫组化。结果所有雌激素均能诱导ABCG 2 mRNA的表达，且呈浓度依赖性。Western blot分析显示17-β-雌二醇诱导ABCG 2蛋白表达。蛋白质印迹分析的质膜部分和免疫组化表明，ABCG 2定位在BeWo细胞的顶端侧，17-β-雌二醇对ABCG 2的定位没有影响。此外，孕酮在1-10 μ M时抑制17-β-雌二醇对ABCG 2表达的诱导。结论妊娠期ABCG 2在胎盘中的表达受雌激素和孕激素的调节。
PURPOSE The aim of this study was to elucidate the effects of sex hormones that are secreted during gestation from the placenta on ABCG2 mRNA and protein expression levels by using the placental cell line BeWo. METHODS We investigated the effects of estrogens (estrone, 17-beta-estradiol and estriol) on the expression level of ABCG2 mRNA by RT-PCR. The expression level of ABCG2 protein was analyzed by Western blot analysis. We also investigated the localization of ABCG2 in BeWo cells by Western blot analysis of the plasma membrane fraction and by immunohistochemistry. RESULTS It was found that all estrogens induce the expression of ABCG2 mRNA in a concentration-dependent manner. Furthermore, Western blot analysis showed that 17-beta-estradiol induces the expression of ABCG2 protein. Western blot analysis of the plasma membrane fraction and immunohistochemistry showed that ABCG2 localized on only the apical side of BeWo cells and that 17-beta-estradiol had no effect on the localization of ABCG2. In addition, progesterone suppressed the induction of ABCG2 expression by 17-beta-estradiol at 1-10 microM. CONCLUSION The expression of ABCG2 in the placenta is regulated by estrogen and progesterone during gestation.