Lactotransferrin gene expression in the mouse uterus and mammary gland.
Lactotransferrin gene expression in the mouse uterus and mammary gland.
复制标题
乳转铁蛋白基因在小鼠子宫和乳腺中的表达。
作者：
C. Teng;B. Pentecost;Yu Chen;R. Newbold;E. M. Eddy;J. Mclachlan
小鼠乳腺和子宫在不同生理条件下表达分泌蛋白乳转铁蛋白基因。然而，乳转铁蛋白在未成熟小鼠的子宫中被雌激素以时间和剂量依赖的方式诱导，但不受乳腺中雌激素的影响。成年雌性哺乳期乳汁转铁蛋白在乳腺和子宫中的表达也存在差异。免疫细胞化学检测到分娩后1天子宫上皮细胞中有较高水平的蛋白表达，但免疫反应很快消失。然而，在哺乳期结束时，乳转铁蛋白信息在乳腺中相对丰富。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和Western印迹分析法研究了乳转铁蛋白在不同组织中的存在。用该方法检测到两种形式的免疫反应物质：雌激素性未成熟小鼠子宫腔液和成年雌性小鼠肺、阴道、乳腺、输卵管、脾、淋巴结和子宫匀浆中均有一条70K条带，颌下腺、肾脏、卵巢和上述所有组织均有一条65K条带。脑和十二指肠未检测到免疫反应物质。在假孕小鼠子宫腔液中观察到乳转铁蛋白的短暂出现。这些在不同生理条件下子宫和乳腺中乳转铁蛋白水平的变化表明，该蛋白的表达调节是组织特异性的。
The mouse mammary gland and uterus expressed the gene for the secretory protein lactotransferrin under various physiological conditions. Lactotransferrin, however, was induced by estrogen in a time- and dose-dependent fashion in the uterus of the immature mouse, but was not affected by estrogen in the mammary gland. Differences were also found in the expression of lactotransferrin in mammary glands and uteri of adult females during lactation. A high level of the protein was detected by immunocytochemistry in uterine epithelial cells 1 day after parturition, but immunoreactivity disappeared quickly thereafter. Lactotransferrin message was, however, relatively abundant in the mammary gland at the end of the lactation period. The presence of lactotransferrin in various tissues also was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. Two forms of immunoreactive material was detected by this method; a 70K band was found in uterine luminal fluid from the estrogen-stimulated immature mouse and in homogenates of lung, vagina, mammary gland, oviduct, spleen, lymph node, and uterus of the adult female mouse, and a 65K band was detected in submaxillary gland, kidney, ovary, and all of the above tissues. Brain and duodenum had no detectable immunoreactive material. A transient appearance of lactotransferrin was observed in the uterine luminal fluid of pseudopregnant mice. These changes in the level of lactotransferrin in the uterus and mammary gland under various physiological conditions suggest that the regulation of this protein's expression is tissue specific.