Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device
Effects of electric fields on human mesenchymal stem cell behaviour and morphology using a novel multichannel device
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DOI：
10.1039/c4ib00297k
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Cooper-White, J. J.
中科院分区：
文献类型：
--
作者：
Banks, T. A.;Luckman, P. S. B.;Cooper-White, J. J.
富含胶原蛋白的组织，如骨和软骨，其固有的压电性会导致在施加机械应力时产生微小的内生电场(EFS)。在体内，这些EFS可能会影响细胞迁移，而细胞迁移是伤口愈合的重要组成部分。因此，小型外用EFS在骨折和皮肤创伤中的应用在临床上得到了积极的实践。由于干细胞在骨和软骨愈合中具有显著的再生潜力，以及它们在体内观察到的EFS促进愈合的潜在作用，我们使用一种新型的中通量装置，研究了生理学和生理学EFS对人骨髓间充质干细胞(HBM-MSCs)长达15小时的影响。施加的电场对HBM-MSC的形态和迁移有显著影响；细胞表现出不同程度的转化为高度拉长的表型，取决于EF强度，一致的垂直于EF载体，以及明确的阴极迁移响应EF强度>；=0.5V cm(-1)，观察到最快的迁移速度在1.7到3V cm(-1)之间。我们观察了HBM-MSC供体对供体反应的变异性和对应用EFS的总体耐受性。因此，这项研究证实了HBM-MSCs对施加的EFS有反应，并且它们向阴极的迁移速度是可控的，取决于EF的强度，这为HBM-MSCs的生理学提供了新的见解，并可能为将EFS用于组织工程应用或植入后的体内定向支架定植提供了重要的机会。
The intrinsic piezoelectric nature of collagenous-rich tissues, such as bone and cartilage, can result in the production of small, endogenous electric fields (EFs) during applied mechanical stresses. In vivo, these EFs may influence cell migration, a vital component of wound healing. As a result, the application of small external EFs to bone fractures and cutaneous wounds is actively practiced clinically. Due to the significant regenerative potential of stem cells in bone and cartilage healing, and their potential role in the observed improved healing in vivo post applied EFs, using a novel medium throughput device, we investigated the impacts of physiological and aphysiological EFs on human bone marrow-derived mesenchymal stem cells (hBM-MSCs) for up to 15 hours. The applied EFs had significant impacts on hBM-MSC morphology and migration; cells displayed varying degrees of conversion to a highly elongated phenotype dependent on the EF strength, consistent perpendicular alignment to the EF vector, and definitive cathodal migration in response to EF strengths >= 0.5 V cm(-1), with the fastest migration speeds observed at between 1.7 and 3 V cm(-1). We observed variability in hBM-MSC donor-to-donor responses and overall tolerances to applied EFs. This study thus confirms hBM-MSCs are responsive to applied EFs, and their rate of migration towards the cathode is controllable depending on the EF strength, providing new insight into the physiology of hBM-MSCs and possibly a significant opportunity for the utilisation of EFs in directed scaffold colonisation in vitro for tissue engineering applications or in vivo post implantation.