Induction of toll-like receptors by Propionibacterium acnes
Induction of toll-like receptors by Propionibacterium acnes
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DOI：
10.1111/j.1365-2133.2005.06933.x
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发表时间：
2005-12-01
影响因子：
10.3
通讯作者：
Dreno, B
中科院分区：
文献类型：
--
作者：
Jugeau, S;Tenaud, I;Dreno, B
痤疮丙酸杆菌参与痤疮炎症期的诱导和维持。最近的研究发现，角质形成细胞表达toll样受体（TLRs）与即时免疫有关。迄今为止，尚无关于痤疮假单胞菌对角质形成细胞中TLR活化作用的研究。目的以痤疮炎症期为研究对象，通过诱导角质形成细胞表达TLR-2和TLR-4，阐明痤疮P. acnes在即刻免疫中的作用。我们还研究了痤疮芽孢杆菌诱导基质金属蛋白酶(MMP)-9的分泌和表达。方法在体内研究痤疮病变活检组织中TLR-2和TLR-4蛋白的表达，在体外培养角质细胞单层上研究痤疮假单胞菌对TLR-2和TLR-4的表达以及MMP-9的表达和分泌的调节作用。结果体内TLR-2和TLR-4的表达在痤疮病变表皮中升高。在体外实验中，人角质形成细胞TLR-2和TLR-4的表达在细菌组分孵育的最初几个小时内增加，角质形成细胞的MMP-9的表达和分泌也增加，而MMP-9在炎症中起作用。结论痤疮假芽胞杆菌诱导TLR表达，该机制可能在痤疮相关炎症中发挥重要作用。这些受体可能与急性痤疮有关。
Background The bacterium Propionibacterium acnes is involved in the induction and maintenance of the inflammatory phase of acne. Recent studies have found that keratinocytes express toll-like receptors (TLRs) implicated in immediate immunity. No studies have, to date, been carried out on the action of P. acnes upon TLR activation in keratinocytes.Objectives Focusing on the inflammatory phase of acne, to clarify the role of P. acnes in immediate immunity by inducing expression of TLR-2 and TLR-4 by keratinocytes. We also studied how the secretion and expression of matrix metalloproteinase (MMP)-9 is induced by P. acnes.Methods The work was carried out on two levels: in vivo with the study of the expression of TLR-2 and TLR-4 proteins in biopsies of acne lesions and in vitro on cultured keratinocyte monolayers to study the modulating effects of P. acnes on the expression of TLR-2 and TLR-4 and also on the expression and secretion of MMP-9.Results Our findings reveal that in vivo TLR-2 and TLR-4 expression is increased in the epidermis of acne lesions. In vitro, an increase in TLR-2 and TLR-4 expression by human keratinocytes occurred in the first hours of incubation with bacterial fractions as well as an increase of the expression and secretion by the keratinocytes of MMP-9, which plays a role in inflammation.Conclusions This work demonstrates that P. acnes induces TLR expression and that this mechanism could play an essential role in acne-linked inflammation. These receptors could be involved notably in acute acne.