Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus
Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus
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猪白细胞抗原2与口蹄疫病毒Asia1血清型CTL表位AS64复合的表达、纯化、结晶及初步X射线衍射分析
DOI：
10.1186/s12917-018-1742-3
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发表时间：
2018-12-18
影响因子：
2.6
通讯作者：
Hu, Xiao
中科院分区：
文献类型：
--
作者：
Gao, Feng-shan;Feng, Lei;Hu, Xiao
背景目前，猪主要组织相容性复合体（MHC）I类分子，又称猪白细胞抗原I类（SLA-I）分子的结构特征有待进一步阐明。结果SLA-1分子与猪（2）-微球蛋白和细胞毒性T淋巴细胞（CTL）表位FMDV-AS64（ALLRSATYY）构成SLA-I复合物（ALLRSATYY）表达、重折叠、纯化和结晶源自口蹄疫病毒（FMDV）亚洲1血清型的VP1蛋白（残基64-72）。初步X射线衍射分析表明，该晶体的衍射分辨率为2.4埃，空间群属于P2(1)2(1)2(1)，晶胞参数a=48.37，b=97.75，c=166.163埃。结论本研究将有利于阐明与亚洲CTL表位相关的SLA-2分子的结构特征1 FMDV 血清型。
BackgroundCurrently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified.ResultsA complex of SLA-I constituted by an SLA-2*HB01 molecule with swine (2)-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64-72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was expressed, refolded, purified and crystallized. By preliminary X-ray diffraction analysis, it was shown that the diffraction resolution of the crystal was 2.4 angstrom and the space group belonged to P2(1)2(1)2(1) with unit cell parameters a=48.37, b=97.75, c=166.163 angstrom.ConclusionThis research will be in favor of illuminating the structural characteristics of an SLA-2 molecule associated with a CTL epitope derived from Asia1 serotype of FMDV.