Full field-of-view virtual reality goggles for mice
Full field-of-view virtual reality goggles for mice
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鼠标用全视场虚拟现实护目镜
DOI：
10.1016/j.neuron.2023.11.019
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发表时间：
2023-12-20
期刊：
影响因子：
16.2
通讯作者：
Dombeck，Daniel A.
中科院分区：
文献类型：
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作者：
Pinke，Domonkos;Issa，John B.;Dombeck，Daniel A.
头部固定小鼠的视觉虚拟现实（VR）系统在研究行为背后的神经回路方面比现实世界的研究具有优势。然而，目前的VR方法不能完全覆盖小鼠的视野，不能立体地照亮双眼区域，并且使实验室框架可见。为了克服这些限制，我们开发了iMRSIV（微型啮齿动物立体照明VR）-用于小鼠的VR护目镜。我们的系统结构紧凑，分别照亮每只眼睛的立体视觉，并为每只眼睛提供180°的视野，从而排除了实验室框架，同时适应扫视。使用iMRSIV的小鼠在导航时比当前基于监视器的系统更快地参与虚拟行为，并对头顶上隐约可见的刺激表现出冻结和逃跑反应。使用iMRSIV与双光子功能成像，我们发现了大量的海马位置细胞在虚拟导航，全球重新映射在环境变化过程中，和独特的反应，位置细胞合奏开销迫在眉睫的刺激。
Visual virtual reality (VR) systems for head-fixed mice offer advantages over real-world studies for investigating the neural circuitry underlying behavior. However, current VR approaches do not fully cover the visual field of view of mice, do not stereoscopically illuminate the binocular zone, and leave the lab frame visible. To overcome these limitations, we developed iMRSIV (Miniature Rodent Stereo Illumination VR)—VR goggles for mice. Our system is compact, separately illuminates each eye for stereo vision, and provides each eye with an ∼180° field of view, thus excluding the lab frame while accommodating saccades. Mice using iMRSIV while navigating engaged in virtual behaviors more quickly than in a current monitor-based system and displayed freezing and fleeing reactions to overhead looming stimulation. Using iMRSIV with two-photon functional imaging, we found large populations of hippocampal place cells during virtual navigation, global remapping during environment changes, and unique responses of place cell ensembles to overhead looming stimulation.