Targeted myocardial microinjections of a biocomposite material reduces infarct expansion in pigs.
Targeted myocardial microinjections of a biocomposite material reduces infarct expansion in pigs.
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DOI：
10.1016/j.athoracsur.2008.04.107
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发表时间：
2008-10
影响因子：
4.6
通讯作者：
Spinale, Francis G.
中科院分区：
文献类型：
--
作者：
Mukherjee, Rupak;Zavadzkas, Juozas A.;Saunders, Stuart M.;McLean, Julie E.;Jeffords, Laura B.;Beck, Christy;Stroud, Robert E.;Leone, Allyson M.;Koval, Christine N.;Rivers, William T.;Basu, Shubhayu;Sheehy, Alexander;Michal, Gene;Spinale, Francis G.
心肌梗死（MI）后左心室（LV）重构通常导致梗死扩大（IE）。本研究试图通过将生物相容性复合材料显微注射到MI后心肌中来中断IE。在21只猪中建立MI（冠状动脉结扎）。放置不透射线标记（2 mm直径）进行IE（荧光透视）。MI后7天，将猪随机进行纤维蛋白-藻酸盐复合物（Fib-Alg;纤维蛋白原、纤连蛋白、因子XIII、明胶接枝藻酸盐、凝血酶; n = 11）或生理盐水（n = 10）的显微注射（25次注射; 2 × 2 cm阵列; 200 µL/注射）。注射后7天（MI后14天），Fib-Alg组的LV后壁厚度高于生理盐水组（分别为1.07 ± 0.11 vs 0.69 ± 0.07 cm，p = 0.002）。在MI后28天，在盐水组（1.71 ± 0.13 cm 2，p = 0.010）和Fib-Alg组（1.44 ± 0.23 cm 2，p < 0.001）中，标记物（IE）内的面积从基线（1 cm 2）增加。然而，在MI后21天和28天，Fib-Alg组中IE的变化降低（p=0.043和p=0.019）。在盐水和Fib-Alg组中，MI区域内的总胶原蛋白含量相似（分别为12.8 ± 1.7和11.6 ± 1.5 µg/mg，p = NS）。然而，Fib-Alg组中可提取的胶原蛋白（指示溶解度）低于生理盐水组（59.1 ± 3.5 vs 71.0 ± 6.1 µg/mL，p = 0.020）。靶向心肌微量注射生物复合物可减轻心肌梗死后左室壁厚度的减少和梗死范围的扩大。因此，术中微量注射生物相容性材料可能为中断MI后LV重塑提供一种新的方法。
Left ventricular (LV) remodeling after myocardial infarction (MI) commonly causes infarct expansion (IE). This study sought to interrupt IE through microinjections of a biocompatible composite material into the post-MI myocardium. MI was created in 21 pigs (coronary ligation). Radiopaque markers (2-mm diameter) were placed for IE (fluoroscopy). Pigs were randomized for microinjections (25 injections; 2- × 2-cm array; 200 µL/injection) at 7 days post-MI of a fibrin-alginate composite (Fib-Alg; fibrinogen, fibronectin, factor XIII, gelatin-grafted alginate, thrombin; n = 11) or saline (n = 10). At 7 days after injection (14 days post-MI), LV posterior wall thickness was higher in the Fib-Alg group than in the saline group (1.07 ± 0.11 vs 0.69 ± 0.07 cm, respectively, p = 0.002). At 28 days post-MI, the area within the markers (IE) increased from baseline (1 cm2) in the saline (1.71 ± 0.13 cm2, p = 0.010) and Fib-Alg groups (1.44 ± 0.23 cm2, p < 0.001). However, the change in IE at 21 and 28 days post-MI was reduced in the Fib-Alg group (p=0.043 and p=0.019). Total collagen content within the MI region was similar in the saline and Fib-Alg groups (12.8 ± 1.7 and 11.6 ± 1.5 µg/mg, respectively, p = NS). However, extractable collagen, indicative of solubility, was lower in the Fib-Alg group than the saline group (59.1 ± 3.5 vs 71.0 ± 6.1 µg/mL, p = 0.020). Targeted myocardial microinjection of the biocomposite attenuated the post-MI decrease in LV wall thickness and infarct expansion. Thus, intraoperative microinjections of biocompatible material may provide a novel approach for interrupting post-MI LV remodeling.
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影响因子：
20.1
作者：
WEBER, KT;JANICKI, JS;BASHEY, RI
通讯作者：
BASHEY, RI
影响因子：
37.8
作者：
Leor, Jonathan;Rozen, Liat;Danon, David
通讯作者：
Danon, David
DOI：
10.1152/ajpheart.00233.2002
发表时间：
2003-01-01
影响因子：
4.8
作者：
Baicu, CF;Stroud, JD;Zile, MR
通讯作者：
Zile, MR
影响因子：
37.8
作者：
Kelley, ST;Malekan, R;Edmunds, LH
通讯作者：
Edmunds, LH
DOI：
10.1152/ajpheart.01343.2005
发表时间：
2006-11-01
影响因子：
4.8
作者：
Mukherjee, Rupak;Mingoia, Joseph T.;Spinale, Francis G.
通讯作者：
Spinale, Francis G.