Effects of Post-Treatment Hydrogen Gas Inhalation on Uveitis Induced by Endotoxin in Rats.
Effects of Post-Treatment Hydrogen Gas Inhalation on Uveitis Induced by Endotoxin in Rats.
复制标题
治疗后氢气吸入对内毒素所致大鼠葡萄膜炎的影响
DOI：
10.12659/msm.907269
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发表时间：
2018-06-07
期刊：
影响因子：
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通讯作者：
Zhang Z
中科院分区：
文献类型：
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作者：
Yan W;Chen T;Long P;Zhang Z;Liu Q;Wang X;An J;Zhang Z
分子氢（H2）已被广泛报道，通过减少氧化应激和炎症，在各种动物模型和人类疾病中具有有益作用。本研究的目的是探讨氢气是否可以改善大鼠内毒素诱导的葡萄膜炎（EIU）。雄性SD大鼠分为正常组、模型组、氮氧（N-O）组和氢氧（H-O）组。后3组大鼠注射脂多糖（LPS）诱导EIU。然后，N-O组吸入67%N2和33%O2的混合气体，H-O组吸入67%H2和33%O2的混合气体。根据视网膜电图（ERG）检查后的葡萄膜炎体征对所有大鼠进行分级。测量房水中的蛋白质浓度（AqH）。此外，进行苏木精-伊红染色和虹膜和睫状体（ICB）中抗离子钙结合衔接分子1（Iba 1）的免疫染色。模型组、N-O组和H-O组大鼠葡萄膜炎分级评分和暗适应3.0 ERG b波峰值时间差异无统计学意义（P>0.05），H-O组AqH蛋白浓度低于模型组和N-O组（P<0.05）。H-O组大鼠ICB内浸润细胞数与模型组和N-O组相比无显著性差异（P>0.05），而小胶质细胞活化程度有所降低（P<0.05）。治疗后吸入氢气并不能改善临床症状，也不能减少EIU的浸润细胞。然而，它抑制了AqH中蛋白质的升高并减少了小胶质细胞的激活。
Molecular hydrogen (H2) has been widely reported to have benefiicial effects in diverse animal models and human disease through reduction of oxidative stress and inflammation. The aim of this study was to investigate whether hydrogen gas could ameliorate endotoxin-induced uveitis (EIU) in rats. Male Sprague-Dawley rats were divided into a normal group, a model group, a nitrogen-oxygen (N-O) group, and a hydrogen-oxygen (H-O) group. EIU was induced in rats of the latter 3 groups by injection of lipopolysaccharide (LPS). After that, rats in the N-O group inhaled a gas mixture of 67% N2 and 33% O2, while those in the H-O group inhaled a gas mixture of 67% H2 and 33% O2. All rats were graded according to the signs of uveitis after electroretinography (ERG) examination. Protein concentration in the aqueous humor (AqH) was measured. Furthermore, hematoxylin-eosin staining and immunostaining of anti-ionized calcium-binding adapter molecule 1 (Iba1) in the iris and ciliary body (ICB) were carried out. No statistically significant differences existed in the graded score of uveitis and the b-wave peak time in the Dark-adapted 3.0 ERG among the model, N-O, and H-O groups (P>0.05), while rats of the H-O group showed a lower concentration of AqH protein than that of the model or N-O group (P<0.05). The number of the infiltrating cells in the ICB of rats from the H-O group was not significantly different from that of the model or N-O group (P>0.05), while the activation of microglia cells in the H-O group was somewhat reduced (P<0.05). Post-treatment hydrogen gas inhalation did not ameliorate the clinical signs, or reduce the infiltrating cells of EIU. However, it inhibited the elevation of protein in the AqH and reduced the microglia activation.