ESTABLISHMENT OF A LINE OF CELLS DERIVED FROM OVARIAN TISSUE OF CULEX-QUINQUEFASCIATUS SAY
ESTABLISHMENT OF A LINE OF CELLS DERIVED FROM OVARIAN TISSUE OF CULEX-QUINQUEFASCIATUS SAY
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DOI：
10.1093/jmedent/7.6.703
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发表时间：
1970-01-01
影响因子：
2.1
通讯作者：
CROSS, JH
中科院分区：
文献类型：
--
作者：
HSU, SH;MAO, WH;CROSS, JH
建立了致倦库蚊成虫卵巢组织来源的蚊虫细胞系，并在体外成功生长。细胞形态为上皮样，牢固附着于血管表面，形成单层。观察到两种不同类型的细胞，纺锤形和圆形。从1969年1月至12月进行了93代连续传代培养。用胎牛血清代替昆虫血淋巴。该品系中的大多数细胞含有二倍体的染色体(n为3)。
A line of mosquito cells derived from ovarian tissue of adultCulex quinquefasciatus(Say) was established and grown successfully in vitro. The cells were epithelioid in appearance and firmly attached to the vessel surface, forming monolayers. Two distinct types of cells were observed, spindle-shaped and round. Serial subcultivation of the cells was carried through 93 passages from January to December 1969. The culture medium contained fetal bovine serum in place of insect hemolymph. Most of the cells in this line contained a diploid number of chromosomes (n is 3).