EFFECT OF DIFFERENT PURINE AUXOTROPHIC MUTATIONS ON MOUSE-VIRULENCE OF A VI-POSITIVE STRAIN OF SALMONELLA-DUBLIN AND OF 2 STRAINS OF SALMONELLA-TYPHIMURIUM
EFFECT OF DIFFERENT PURINE AUXOTROPHIC MUTATIONS ON MOUSE-VIRULENCE OF A VI-POSITIVE STRAIN OF SALMONELLA-DUBLIN AND OF 2 STRAINS OF SALMONELLA-TYPHIMURIUM
复制标题
DOI：
10.1016/0882-4010(87)90071-4
复制
发表时间：
1987-08-01
影响因子：
3.8
通讯作者：
STOCKER, BAD
中科院分区：
文献类型：
--
作者：
MCFARLAND, WC;STOCKER, BAD
将10 cfu的都柏林沙门氏菌Vi阳性菌株腹膜内给予BALB/c小鼠，导致死亡。在预期具有降低毒力的营养需求的转导衍生物中，在aroA（因此是芳香族依赖性的）或purA（因此是腺嘌呤依赖性的）处具有完全阻断的那些是无毒力的;分别从106或107 cfu的i. p.接种物中没有死亡。在purF、purG、purC或purJHD操纵子中具有确定的块的转导子，所有次黄嘌呤或任何其他嘌呤都具有满足嘌呤要求的嘌呤，具有降低的毒力，一些死亡从104 cfu开始，或者对于大多数衍生物，从仅102 cfu开始。阻断guaA或guaB，导致鸟嘌呤需求，同样只导致毒力降低，102接种物导致一些死亡。两种小鼠毒性的转导衍生物（LD 50，i. p.，< 25 cfu）的S.类似地测试了鼠伤寒沙门氏菌，结果大体相似，因为purA和purB（需要腺嘌呤的）转导子是无毒的，2.5 × 10 - 6鼠伤寒沙门氏菌没有死亡。107 cfu，而purF、purG或purC缺陷（次黄嘌呤应答）的菌株保留部分毒力。然而，guaA和guaB（需要鸟嘌呤的）衍生物的毒力大大降低;所有小鼠存活2.5倍。105 cfu，但只有一个存活了2.5 × 105 cfu。107 cfu。在S.都柏林和S.鼠伤寒试验尸检培养物表明，致命感染是由营养缺陷型菌株的增殖引起的，而不是由非严格回复突变体的增殖引起的。
Ten cfu of a Vi-positive strain of Salmonella dublin, given i.p. to BALB/c mice, caused death. Of transductional derivatives with nutritional requirements expected to reduce virulence, those with complete blocks at aroA (therefore aromatic-dependent) or a purA (therefore adenine-dependent) were non-virulent; no deaths from i.p. inocula of 106 or 107 cfu, respectively. Transductants with identified blocks at purF, purG, purC or in the purJHD operon, all with purine requirement satisfied by hypoxanthines or any other purine, were of reduced virulence, with some deaths from 104 cfu or, for most of the derivatives, from only 102 cfu. Blocks at guaA or guaB, causing guanine requirement, likewise resulted only in reduced virulence, with some deaths from inocula of 102. Transductional derivatives of two mouse-virulent (LD50, i.p., < 25 cfu) strains of S. typhimurium were similarly tested, with generally similar results, in that purA and purB (adenine-requiring) transductants were non-virulent, no deaths from 2.5 .times. 107 cfu, and those with purF, purGor purC defects (hypoxanthine-responding) retained partial virulence. However, the guaA and guaB (guanine-requiring) derivatives were of much reduced virulence; all mice survived 2.5 .times. 105 cfu but only one survived 2.5 .times. 107 cfu. In both the S. dublin and the S. typhimurium tests autopsy cultures showed that fatal infections resulted from multiplication of the auxotrophic strain, not from that of non-exacting revertants.