Milk Fat Globule Membrane Supplementation in Formula Modulates the Neonatal Gut Microbiome and Normalizes Intestinal Development.
Milk Fat Globule Membrane Supplementation in Formula Modulates the Neonatal Gut Microbiome and Normalizes Intestinal Development.
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DOI：
10.1038/srep45274
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发表时间：
2017-03-28
影响因子：
4.6
通讯作者：
Vallance BA
中科院分区：
文献类型：
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作者：
Bhinder G;Allaire JM;Garcia C;Lau JT;Chan JM;Ryz NR;Bosman ES;Graef FA;Crowley SM;Celiberto LS;Berkmann JC;Dyer RA;Jacobson K;Surette MG;Innis SM;Vallance BA
母乳具有许多有益的特性和不寻常的特征，包括独特的脂肪成分，称为乳脂球膜（MFGM）。虽然母乳对发育有重要的益处，但在有些情况下，母乳无法获得，导致需要配方奶粉喂养。大多数配方奶粉不含MFGM，但其脂质来自植物来源，其大小和组成差异很大。在这里，我们测试了MFGM补充剂对肠道发育和微生物组的影响，以及其预防艰难梭菌诱导的结肠炎的潜力。使用杯中幼鼠模型向5至15日龄的大鼠递送对照或添加MFGM的配方食品;使用母乳（MM）饲养的动物作为对照。虽然与MM同窝仔相比，CTL配方在肠道发育方面产生显著缺陷，但向配方中添加MFGM使肠道生长、潘氏细胞和杯状细胞数量以及紧密连接蛋白模式恢复至MM幼仔的水平。此外，MFGM和MM幼仔的肠道微生物群显示出比CTL更大的相似性，并证明对C。艰难梭菌毒素诱导炎症。因此，我们的研究表明，在配方奶粉中添加MFGM可促进肠上皮和微生物组的发育，并防止炎症。
Breast milk has many beneficial properties and unusual characteristics including a unique fat component, termed milk fat globule membrane (MFGM). While breast milk yields important developmental benefits, there are situations where it is unavailable resulting in a need for formula feeding. Most formulas do not contain MFGM, but derive their lipids from vegetable sources, which differ greatly in size and composition. Here we tested the effects of MFGM supplementation on intestinal development and the microbiome as well as its potential to protect against Clostridium difficile induced colitis. The pup-in-a-cup model was used to deliver either control or MFGM supplemented formula to rats from 5 to 15 days of age; with mother’s milk (MM) reared animals used as controls. While CTL formula yielded significant deficits in intestinal development as compared to MM littermates, addition of MFGM to formula restored intestinal growth, Paneth and goblet cell numbers, and tight junction protein patterns to that of MM pups. Moreover, the gut microbiota of MFGM and MM pups displayed greater similarities than CTL, and proved protective against C. difficile toxin induced inflammation. Our study thus demonstrates that addition of MFGM to formula promotes development of the intestinal epithelium and microbiome and protects against inflammation.