BPTI liquid-liquid phase separation monitored by light and small angle X-ray scattering.
BPTI liquid-liquid phase separation monitored by light and small angle X-ray scattering.
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通过光和小角 X 射线散射监测 BPTI 液-液相分离。
DOI：
10.1107/s0907444902012799
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Veesler
中科院分区：
文献类型：
--
作者：
S. Grouazel;J. Pérez;J. Astier;F. Bonneté;S. Veesler
在蛋白质结晶领域，更好地了解成核过程对于控制成核速率、生长以及晶体的尺寸和质量至关重要。有了这个目标，很明显，重要的阶段是确定蛋白质相图。我们强调并研究了牛胰胰蛋白酶抑制剂（BPTI）二元液-液相分离在350 mM KSCN溶液中作为温度的函数。我们用光散射和光学显微镜测量了双节曲线的低浓度部分。我们表明，从小角度的X-射线散射实验，高浓度的相沉积在毛细管的底部，我们分析了低浓度相的单体/十聚体平衡。
In the field of protein crystallization, a better knowledge of the nucleation process is essential to control the nucleation rate, the growth and therefore the size and the quality of crystals. With that aim, it becomes clear that the important stage is the determination of the protein phase diagram. We highlighted and investigated the bovine pancreatic trypsin inhibitor (BPTI) binary liquid-liquid phase separation in 350 mM KSCN solutions as a function of temperature. We measured the low concentration part of the binodal curve using light scattering and optical microscopy. We show, from small angle X-ray scattering experiments, that the high concentrated phase sediments in the bottom of the capillary and we analysed the low concentrated phase in terms of monomers/decamers equilibrium.