Regional difference in corticotropin-releasing factor immunoreactivity in mossy fiber terminals innervating calretinin-immunoreactive unipolar brush cells in vestibulocerebellum of rolling mouse Nagoya
Regional difference in corticotropin-releasing factor immunoreactivity in mossy fiber terminals innervating calretinin-immunoreactive unipolar brush cells in vestibulocerebellum of rolling mouse Nagoya
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DOI：
10.1016/j.brainres.2005.09.018
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发表时间：
2005-11-23
期刊：
影响因子：
2.9
通讯作者：
Fukui, Y
中科院分区：
文献类型：
--
作者：
Ando, M;Sawada, K;Fukui, Y
单极刷细胞（ubc）是一类前庭小脑的中间神经元，在放大来自前庭小脑苔藓纤维的兴奋输入中起作用。本研究旨在阐明在名古屋滚动小鼠（RMN）中，促肾上腺皮质激素释放因子（CRF）阳性的calretinin （CR）阳性ubc的苔藓纤维神经支配是否发生改变。RMN小鼠前庭小脑中cr阳性ubc的分布和数量与对照组无明显差异。双免疫荧光显示一些crf阳性的苔藓纤维末端与cr阳性的ubc密切相关。在蚯蚓的X小叶中，这种苔藓状纤维末端的数量在RMN组是对照组的5倍左右。相比之下，小叶中毗邻cr阳性UBCs的crf阳性苔藓纤维终端的数量在RMN和对照组之间没有显著差异。结果表明，在RMN的X小叶中，支配cr阳性UBCs的crf阳性苔藓纤维终端数量增加，而在小叶中则没有。CRF可能改变RMN X小叶中cr阳性ubc介导的兴奋通路，并可能干扰X小叶的功能，如小脑对头部线性运动的适应。(c) 2005 Elsevier B.V.版权所有
Unipolar brush cells (UBCs), a class of interneurons in the vestibulocerebellum, play roles in amplifying excitatory inputs from vestibulocerebellar mossy fibers. This study aimed to clarify whether corticotropin-releasing factor (CRF)-positive mossy fiber innervation of calretinin (CR)-positive UBCs was altered in rolling mouse Nagoya (RMN). The distribution and the number of CR-positive UBCs in the vestibulocerebellum were not different between RMN and control mice. Double immunofluorescence revealed that some CRF-positive mossy fiber terminals were in close apposition to CR-positive UBCs. In the lobule X of vermis, such mossy fiber terminals were about 5-fold greater in number in RMN than in controls. In contrast, the number of CRF-positive mossy fiber terminals adjoining CR-positive UBCs in the flocculus was not significantly different between RMN and controls. The results suggest increased number of CRF-positive mossy fiber terminals innervating CR-positive UBCs in the lobule X but not in the flocculus of RMN. CRF may alter CR-positive UBC-mediated excitatory pathways in the lobule X of RMN and may disturb functions of the lobule X such as cerebellar adaptation for linear motion of the head. (c) 2005 Elsevier B.V. All rights reserved.